Abstract
Restriction endonuclease maps of some trypanosome variant surface glycoprotein (VSG) genes have a site 3′ to the gene where many enzymes appear to cut. This site is sensitive to Bal31 exonuclease, indicating a natural double-strand break in the DNA. One VSG gene exists in several cell clones as a non-integrated minichromosome.
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Williams, R., Young, J. & Majiwa, P. Genomic environment of T. brucei VSG genes: presence of a minichromosome. Nature 299, 417–421 (1982). https://doi.org/10.1038/299417a0
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DOI: https://doi.org/10.1038/299417a0
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