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Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions

Nature volume 299, pages 312–316 (1982)Cite this article

Abstract

In physiological ionic conditions (200mM NaCl), the (dC–dG)16 and (dC–dG)13 blocks in plasmid pRW751 are in a left-handed state when the negative superhelical density of the plasmid is greater than 0.072. As the salt concentration decreases or when (dmC–dG) sequences are present, less negative supercoiling is required to induce the right- to left-handed DNA transition. Furthermore, the single strand-specific nuclease, S1, recognizes and cleaves aberrant structural features at the junction between neighbouring right- and left-handed DNA regions.

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References

  1. Klysik, J., Stirdivant, S. M., Larson, J. E., Hart, P. A. & Wells, R. D. Nature 290, 672–677 (1981).

    Article  ADS  CAS  Google Scholar 

  2. Stirdivant, S. M., Klysik, J. & Wells, R. D. J. biol. Chem. (in the press).

  3. Zacharias, W., Larson, J. E., Klysik, J., Stirdivant, S. M. & Wells, R. D. J. biol. Chem. 257, 2775–2782 (1982).

    CAS  PubMed  Google Scholar 

  4. Pulleyblank, D. E., Shure, M., Tang, D., Vinograd, J. & Vosberg, H.-P. Proc. natn. Acad. Sci. U.S.A. 72, 4280–4284 (1975).

    Article  ADS  CAS  Google Scholar 

  5. Depew, R. E. & Wang, J. C. Proc. natn. Acad. Sci. U.S.A. 72, 4275–4279 (1975).

    Article  ADS  CAS  Google Scholar 

  6. Panayotatos, N. & Wells, R. D. Nature 289, 446–470 (1981).

    Article  ADS  Google Scholar 

  7. Lilley, D. M. J. Proc. natn. Acad. Sci. U.S.A. 77, 6468–6472 (1980).

    Article  ADS  CAS  Google Scholar 

  8. Singleton, C. K. & Wells, R. D. J. biol. Chem. 257, 6292–6295 (1982).

    CAS  PubMed  Google Scholar 

  9. Klysik, J., Stirdivant, S. M. & Wells, R. D. J. biol. Chem (in the press).

  10. Singleton, C. K. & Wells, R. D. Analyt. Biochem. 122, 253–257 (1982).

    Article  CAS  Google Scholar 

  11. Tinoco, I., Uhlenbeck, O. C. & Levine, M. D. Nature 230, 362–367 (1971).

    Article  ADS  CAS  Google Scholar 

  12. Haasnoot, C. A. G., den Hartog, J. H. J., de Rooij, J. F. M., van Boom, J. H. & Altona, C. Nucleic Acids Res. 8, 169–181 (1980).

    Article  CAS  Google Scholar 

  13. Wells, R. D. et al. J. biol. Chem (in the press).

  14. Drew, H. R. & Dickerson, R. E. J. molec. Biol. 152, 723–736 (1981).

    Article  CAS  Google Scholar 

  15. Wang, J. C. J. molec. Biol. 89, 783–801 (1974).

    Article  CAS  Google Scholar 

  16. Wang, J. C. Trends biochem. Sci. 5, 219–221 (1980).

    Article  CAS  Google Scholar 

  17. Benham, C. J. J. molec. Biol. 150, 43–68 (1981).

    Article  CAS  Google Scholar 

  18. Camerini-Otero, R. D. & Felsenfeld, G. Proc. natn. Acad. Sci. U.S.A. 75, 1708–1712 (1978).

    Article  ADS  CAS  Google Scholar 

  19. Vologodski, A. V., Anshelevich, V. V., Lukashin, A. V. & Frank-Kamenetskii, M. D. Nature 280, 294–298 (1979).

    Article  ADS  Google Scholar 

  20. Bauer, W. R. A. Rev. Biophys. Bioengng 7, 287–313 (1978).

    Article  CAS  Google Scholar 

  21. Keller, W. Proc. natn. Acad. Sci. U.S.A. 72, 4876–4880 (1975).

    Article  ADS  CAS  Google Scholar 

  22. Behe, M., & Felsenfeld, G. Proc. natn. Acad. Sci. U.S.A. 78, 1619–1623 (1981).

    Article  ADS  CAS  Google Scholar 

  23. Behe, M., Zimmerman, S. & Felsenfeld, G. Nature 293, 233–235 (1981).

    Article  ADS  CAS  Google Scholar 

  24. Wartell, R. M., Klysik, J., Hillen, W. & Wells, R. D. Proc. natn. Acad. Sci. U.S.A. 79, 2549–2553 (1982).

    Article  ADS  CAS  Google Scholar 

  25. Gellert, M. A. Rev. Biochem. 50, 879–910 (1981).

    Article  CAS  Google Scholar 

  26. Pettijohn, D. E. & Pfinninger, O. Proc. natn. Acad. Sci. U.S.A. 77, 1331–1335 (1980).

    Article  ADS  CAS  Google Scholar 

  27. Sinden, R. R., Carlson, J. O. & Pettijohn, D. E. Cell 21, 773–783 (1980).

    Article  CAS  Google Scholar 

  28. Dodgson, J. B. & Wells, R. D. Biochemistry 16, 2374–2379 (1977).

    Article  CAS  Google Scholar 

  29. Southern, E. M. Analyt. Biochem. 100, 319–323 (1979).

    Article  CAS  Google Scholar 

  30. Mann, M. B. & Smith, H. O. in Transmethylation (eds Usdin, E., Borchardt, R. T. & Creveling, C. R.) 483–492 (Elsevier, New York, 1979).

    Google Scholar 

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Author notes

  1. Charles K. Singleton, Jan Klysik & Robert D. Wells

    Present address: Schools of Medicine and Dentistry, Department of Biochemistry, University of Alabama, Birmingham, Birmingham, Alabama, 35294, USA

  2. Steven M. Stirdivant

    Present address: The Biological Laboratories, Harvard University, Cambridge, Massachusetts, 02138, USA

Authors and Affiliations

  1. Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin–Madison, Madison, Wisconsin, 53706, USA

    Charles K. Singleton, Jan Klysik, Steven M. Stirdivant & Robert D. Wells

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  1. Charles K. Singleton
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Singleton, C., Klysik, J., Stirdivant, S. et al. Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions. Nature 299, 312–316 (1982). https://doi.org/10.1038/299312a0

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