Abstract
A wide range of receptors are located at the blood sinusoidal aspect of the hepatocyte plasma membrane1,2. Many circulating ligands that bind to receptors on the cell surfaces are interiorized along two pathways. Asialoglycoproteins3 are transferred from the plasma membrane to lysosomes and degraded, whereas immunoglobulin A4 and bile acids5 are transported across the hepatocyte interior and released into bile. Asialotransferrin type 3 (ref. 6) follows a further pathway termed diacytosis7. After binding to the asialoglycoprotein receptor8, asialotransferrin is endocytosed and then returned to blood with a proportion of its carbohydrate side chains resialylated9. We now describe in liver the properties of intracellular asialotransferrin-enclosing vesicles (diacytosomes) and show that they differ from Golgi, lysosome and plasma membrane fractions. Furthermore, we show that the asialoglycoprotein binding sites are located on the cytoplasmic (outer) surface of diacytosomes.
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Debanne, M., Evans, W., Flint, N. et al. Receptor-rich intracellular membrane vesicles transporting asialotransferrin and insulin in liver. Nature 298, 398–400 (1982). https://doi.org/10.1038/298398a0
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DOI: https://doi.org/10.1038/298398a0
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