Abstract
The proton-translocating ATPases from mitochondria, chloroplasts and bacteria consist of ∼10 different polypeptide subunits1. The smallest subunit, a proteolipid of molecular weight ∼8,000, is present in a stoichiometric amount of six—these six polypeptides are thought to form a proton channel through the membrane2. The proteolipid is affected by the ATPase inhibitors oligomycin and dicyclohexylcarbodiimide (DCCD). As DCCD is covalently bound to it, the proteolipid is commonly referred to as the DCCD-binding protein. Because of the spatial arrangement of the proteolipids in the membrane, binding is probably restricted to one specific glutamic acid residue2,3. In the yeast Saccharomyces cerevisiae the DCCD-binding protein is encoded by the mitochondrial DNA and synthesized inside the mitochondria4, whereas in Neurospora crassa, another ascomycete, the gene for the DCCD-binding protein lies in the nucleus, and the protein is synthesized outside the mitochondria5. Here we report the presence on the mitochondrial DNA of N. crassa of a nucleotide sequence which potentially encodes another DCCD-binding protein. Although a translation product has not yet been found, we suggest a possible function of this gene and speculate on its evolutionary origin.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Boyer, P. D. et al. A. Rev. Biochem. 46, 955–1026 (1977).
Sebald, W., Graf, T. & Lukins, H. B. Eur. J. Biochem. 93, 587–599 (1979).
Sebald, W., Machleidt, W. & Wachter, E. Proc. natn. Acad. Sci. U.S.A. 77, 785–789 (1980).
Orian, J. M., Murphy, M. & Marzuki, S. Biochim. biophys. Acta 652, 234–239 (1981).
Jackl, G. & Sebald, W. Eur. J. Biochem. 54, 97–106 (1975).
Agsteribbe, E., Samallo, J., De Vries, H., Hensgens, L. A. M. & Grivell, L. A. in The Organization and Expression of Mitochondrial Genome (eds Kroon, A. M. & Saccone, C.) 51–60 (Elsevier, Amsterdam, 1980).
Hensgens, L. A. M., Grivell, L. A., Borst, P. & Bos, J. L. Proc. natn. Acad. Sci. U.S.A. 76, 1663–1667 (1979).
Sebald, W., Hoppe, J. & Wachter, E. in Function and Molecular Aspects of Biomembrane Transport (ed. Quagliariello, E.) 63–74 (Elsevier, Amsterdam, 1979).
Martin, S. L., Zimmer, E. A., Kan, Y. W. & Wilson, A. C. Proc. natn. Acad. Sci. U.S.A. 77, 3563–3566 (1980).
Nienhaus, A. W. & Stamatoyannopoulos, G. Cell 15, 307–315 (1978).
Ford, P. J. & Brown, R. D. Cell 8, 485–493 (1976).
Margulis, L. Origin of Eukaryotic Cells (Yale University Press, New Haven, 1970).
Lewin, R. Science 214, 426–429 (1981).
Wenzler, H. & Brambl, R. J. biol. Chem. 256, 7166–7173 (1981).
van den Boogaart, P., Samallo, J. & Agsteribbe, E. Cold Spring Harb. Conf. Mitochondrial Genes (Cold Spring Harbor Laboratories, New York, 1982).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van den Boogaart, P., Samallo, J. & Agsteribbe, E. Similar genes for a mitochondrial ATPase subunit in the nuclear and mitochondrial genomes of Neurospora crassa. Nature 298, 187–189 (1982). https://doi.org/10.1038/298187a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/298187a0
This article is cited by
-
Mitochondrial genomes revisited: why do different lineages retain different genes?
BMC Biology (2024)
-
Retention of functional genes for S19 ribosomal protein in both the mitochondrion and nucleus for over 60 million years
Molecular Genetics and Genomics (2015)
-
Improvement of fluorescent chromosome in situ PCR and its application in the phylogeny of the genus Fagopyrum Mill. using nuclear genes of chloroplast origin (cpDNA)
Plant Systematics and Evolution (2013)
-
Nuclear pseudogenes of mitochondrial DNA as a variable part of the human genome
Cell Research (1999)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.