Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Derivation of macrophage-like lines from the pre-B lymphoma ABLS 8.1 using 5-azacytidine

Nature volume 297, pages 691–693 (1982)Cite this article

Abstract

Variation in the degree of methylation of DNA seems to be one mode of regulating gene expression in eukaryotic cells1–7. The relationship between DNA demethylation and gene activation observed in globin4–6 and viral2 genes, together with evidence that alterations in the degree of DNA methylation of a gene are heritable8, although not with 100% fidelity9, have suggested that this may be a mechanism of control of differentiation. Futhermore, exposure to the demethylating drug 5-azacytidine (5-AC) causes differentiation of 3T3 cells into striated muscle cells, chondrocytes and adipocytes7. Subsequent studies have shown that these effects are due to DNA demethylation10. In view of these observations, we have now attempted to modify several continuous B-cell lines with 5-AC. Following exposure of the pre-B lymphoma ABLS 8.1 to 5-AC, we have derived cloned cell lines which possess macrophage-like characteristics not expressed by ABLS 8.1. Similar macrophage-like cell lines were obtained in two independent experiments; they have been re-cloned and remain stable after 4 months of continuous culture.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Groudine, M. & Weintraub, H. Cell 24, 393–401 (1981).

    Article  CAS  Google Scholar 

  2. Groudine, M., Eisenman, R. & Weintraub, H. Nature 292, 311–317 (1981).

    Article  ADS  CAS  Google Scholar 

  3. Naveh-Mang, T. & Cedar, H. Proc. natn. Acad. Sci. U.S.A. 78, 4246–4249 (1981).

    Article  ADS  Google Scholar 

  4. Wu, C. Nature 284, 856–860 (1980).

    Google Scholar 

  5. van der Ploeg, L. H. T. & Flavell, R. A. Cell 19, 947–958 (1980).

    Article  CAS  Google Scholar 

  6. McGhee, J. D. & Ginder, G. D. Nature 280, 419–420 (1979).

    Article  ADS  CAS  Google Scholar 

  7. Taylor, S. M. & Jones, P. A. Cell 17, 771–779 (1979).

    Article  CAS  Google Scholar 

  8. Pollack, Y., Stein, R., Razin, A. & Cedar, H. Proc. natn. Acad. Sci. U.S.A. 77, 6463–6467 (1980).

    Article  ADS  CAS  Google Scholar 

  9. Wigler, M., Levy, D. & Perucho, M. Cell 24, 33–38 (1981).

    Article  CAS  Google Scholar 

  10. Jones, P. A. & Taylor, S. M. Cell 20, 85–93 (1980).

    Article  CAS  Google Scholar 

  11. Sklar, M. D., Shevach, E. M., Green, I. & Potter, M. Nature 253, 550 (1975).

    Article  ADS  CAS  Google Scholar 

  12. Premkumar, E., Potter, M., Singer, P. A. & Sklar, M. D. Cell 6, 149 (1975).

    Article  CAS  Google Scholar 

  13. Cory, S., Adams, J. M. & Kemp, D. J. Proc. natn. Acad. Sci. U.S.A. 77, 4943–4947 (1980).

    Article  ADS  CAS  Google Scholar 

  14. Wiener, F., Klein, G. & Harris, H. J. Cell Sci. 15, 177–183 (1974).

    CAS  PubMed  Google Scholar 

  15. Ledbetter, J. A. & Herzenberg, L. A. Immun. Rev. 47, 63 (1979).

    Article  CAS  Google Scholar 

  16. Pace, J. L. & Russell, S. W. J. Immun. 126, 1863–1867 (1981).

    CAS  PubMed  Google Scholar 

  17. Kemp, D. J., Harris, A. W., Cory, S. & Adams, J. M. Proc. natn. Acad. Sci. U.S.A. 77, 2876–2880 (1980).

    Article  ADS  CAS  Google Scholar 

  18. Kemp, D. J., Harris, A. W. & Adams, J. M. Proc. natn. Acad. Sci. U.S.A. 77, 7400–7404 (1980).

    Article  ADS  CAS  Google Scholar 

  19. Schrader, J. W., Clark-Lewis, I. & Bartlett, P. F. in Biology of Bone Marrow Transplantation, 443–459 (Academic, New York, 1980).

    Google Scholar 

  20. Burgess, A. W. et al. Expl Haemat. 9, 893–903 (1981).

    CAS  Google Scholar 

  21. Schrader, J. W. & Clark-Lewis, I. J. Immun. 126, 1101–1105 (1981).

    CAS  PubMed  Google Scholar 

  22. Clark-Lewis, I. & Schrader, J. W. J. Immun. 127, 1941–1947 (1981).

    CAS  PubMed  Google Scholar 

  23. Ozato, K., Mayer, N. & Sachs, D. H. J. Immun. 124, 533–540 (1980).

    CAS  Google Scholar 

  24. Springer, T., Galfré, G., Secher, D. S. & Milstein, C. Eur. J. Immun. 9, 301–306 (1979).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Victoria, 3050, Australia

    A. W. Boyd & J. W. Schrader

Authors
  1. A. W. Boyd
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. W. Schrader
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boyd, A., Schrader, J. Derivation of macrophage-like lines from the pre-B lymphoma ABLS 8.1 using 5-azacytidine. Nature 297, 691–693 (1982). https://doi.org/10.1038/297691a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/297691a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing