Abstract
The structure of simian virus 40 (SV40) DNA insertions is different from those of retrovirus proviruses and movable genetic elements. No single DNA sequence in either the cell or the SV40 genome serves as an obligatory site of SV40 integrative recombination1–9, and SV40 DNA insertions are not bordered by the repeat structures characteristic of transposons and retrovirus proviruses10–15. Integration of SV40 could involve the matching of short stretches of homologous sequences present in the otherwise heterologous SV40 and cellular genomes. To explore this possibility, I have now sequenced a rat DNA fragment that contains an unoccupied site of SV40 DNA integration. Comparison of this sequence with that of SV40 and that at the rat-SV40 recombinant junction allowed two conclusions: first, SV40 DNA became linked to rat DNA at a point where the two genomes shared 5 base pairs (bp) of DNA sequence homology; and second, a rearrangement of the rat genome, probably a deletion of at least 3 kilobases (kb), occurred at the site of SV40 integration.
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Stringer, J. DNA sequence homology and chromosomal deletion at a site of SV40 DNA integration. Nature 296, 363–366 (1982). https://doi.org/10.1038/296363a0
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DOI: https://doi.org/10.1038/296363a0
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