Abstract
The presence of endogenous ligands of opiate receptors in gastrointestinal organs has been demonstrated by both bioassay and immunoassay but so far no ligand has been precisely identified. We report here the purification from porcine duodenum of an opioid peptide whose structure coincides with that of the pituitary opioid, dynorphin1. Only the N-terminal tridecapeptide of pituitary dynorphin has been published; we have now determined the complete sequence of duodenal dynorphin, and measured its content in the duodenum by radioimmunoassay2. We report here that the complete heptadecapeptide has almost the same potency as dynorphin1–13 in two bioassays.
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Tachibana, S., Araki, K., Ohya, S. et al. Isolation and structure of dynorphin, an opioid peptide, from porcine duodenum. Nature 295, 339–340 (1982). https://doi.org/10.1038/295339a0
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DOI: https://doi.org/10.1038/295339a0
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