Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Review Article
  • Published:

A new era in mammalian gene mapping: somatic cell genetics and recombinant DNA methodologies

Nature volume 294, pages 115–120 (1981)Cite this article

Abstract

Mammalian gene mapping techniques are now sufficiently advanced to contribute significantly to prenatal diagnosis and to human molecular genetics. Restriction fragment mapping can be used to place polymorphic genetic markers at random sites within the genome, and these sites used to assign genes responsible for disease conditions to a chromosomal region. Somatic cell genetic techniques can then be applied to saturate that region with additional restriction fragment markers, some of which will be closely linked to the disease gene. Closely linked restriction fragment markers, especially flanking pairs of markers, can act as predictors for the transmission of defective genes to offspring. A series of tightly linked flanking restriction markers might in addition contribute to the eventual isolation and cloning of the disease gene itself.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Harris, M. in Cell Culture and Somatic Variation (Holt, Rinehart & Winston, New York, 1964).

    Google Scholar 

  2. Ringertz, N. & Savage, R. in Cell Hybrids (Academic, New York, 1976).

    Google Scholar 

  3. Siminovitch, L. Cell 7, 1–11 (1976).

    Article  CAS  Google Scholar 

  4. Kao, F., Chasin, L. & Puck, T. Proc. natn. Acad. Sci. U.S.A. 64, 1284–1291 (1969).

    Article  ADS  CAS  Google Scholar 

  5. Davidson, R. L. A. Rev. Genet. 8, 195–218 (1974).

    Article  CAS  Google Scholar 

  6. Ruddle, F. H. & Creagan, R. P. A. Rev. Genet. 9, 407–486 (1975).

    Article  CAS  Google Scholar 

  7. McKusick, V. & Ruddle, F. Science 196, 390–405 (1977).

    Article  ADS  CAS  Google Scholar 

  8. Fournier, R. E. K. & Ruddle, F. H. Proc. natn. Acad. Sci. U.S.A. 74, 319–323 (1977).

    Article  ADS  CAS  Google Scholar 

  9. Klobutcher, L. A. & Ruddle, F. H. A Rev. Biochem. 50, 533–554 (1981).

    Article  CAS  Google Scholar 

  10. McBride, O. W. & Ozer, H. L. Proc. natn. Acad. Sci. U.S.A. 70, 1258–1262 (1973).

    Article  ADS  CAS  Google Scholar 

  11. Miller, C. L. & Ruddle, R. H. Proc. natn. Acad. Sci. U.S.A. 75, 3346–3350 (1978).

    Article  ADS  CAS  Google Scholar 

  12. Wigler, M., Silverstein, S., Lee, L-S., Pellicer, F., Cheng, Y-C. & Axel, R. Cell 11, 223–232 (1977).

    Article  CAS  Google Scholar 

  13. Capecchi, M. Cell 22, 479–488 (1980).

    Article  CAS  Google Scholar 

  14. Ruddle, F. H. in Genes, Chromosomes and Neoplasia (eds Arrighi, F. E., Rao, P. N. & Stubblefield, E.) (Ravel, New York, 1981).

    Google Scholar 

  15. Deisseroth, A. et al. Cell 12, 205–218 (1977).

    Article  CAS  Google Scholar 

  16. Deisseroth, A. et al. Proc. natn. Acad. Sci. U.S.A. 75, 1456–1460 (1978).

    Article  ADS  CAS  Google Scholar 

  17. Swan, D. et al. Proc. natn. Acad. Sci. U.S.A. 76, 2735–2739 (1979).

    Article  ADS  CAS  Google Scholar 

  18. D'Eustachio, P., Bothwell, A. L., Takaro, T. T., Baltimore, D. & Ruddle, F. H. J. exp. Med. 153, 793–800 (1981).

    Article  CAS  Google Scholar 

  19. D'Eustachio, P., Pravtcheva, D., Marcu, K. & Ruddle, F. H. J. exp. Med. 151, 1545–1550 (1980).

    Article  CAS  Google Scholar 

  20. Ricciuti, F. & Ruddle, F. H. Genetics 74, 661–678 (1973).

    CAS  PubMed  PubMed Central  Google Scholar 

  21. The Human Genetic Mutant Repository (NIH Publ. 80–2011, 1980).

  22. Bergsma, D. Human Gene Mapping Vols I (1973), II (1974), III (1976), IV (1978), V (1979).

    Google Scholar 

  23. Gusella, J. et al. Proc. natn. Acad. Sci. U.S.A. 76, 5239–5243 (1979).

    Article  ADS  CAS  Google Scholar 

  24. Gray, J. et al Proc. natn. Acad. Sci. U.S.A. 72, 1231–1234 (1975).

    Article  ADS  CAS  Google Scholar 

  25. Lebo, R. V. et al. Proc. natn. Acad. Sci. U.S.A. 76, 5804–5808 (1979).

    Article  ADS  CAS  Google Scholar 

  26. Gall, J. & Pardue, M. L. Proc. natn. Acad. Sci. U.S.A. 63, 378–383 (1969).

    Article  ADS  CAS  Google Scholar 

  27. Robins, D., Ripley, S., Henderson, A. & Axel, R. Cell 23, 29–40 (1981).

    Article  CAS  Google Scholar 

  28. Malcolm, S., Barton, P., Murphy, C. & Ferguson-Smith, M. Ann. Hum. Genet. 45, 135–142 (1981).

    Article  CAS  Google Scholar 

  29. Chandler-Harper, M. E. & Saunders, G. Chromosoma (in the press).

  30. Harper, M., Ullrich, A. & Saunders, G. Proc. natn. Acad. Sci. U.S.A. 78, 4458–4460 (1981).

    Article  ADS  CAS  Google Scholar 

  31. Harper, M., Barrera-Saldana, H. & Saunders, G. Am. J. hum. Genet. (in the press).

  32. Wahl, G., Stern, M. & Stark, G. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).

    Article  ADS  CAS  Google Scholar 

  33. Diaz, M., Barsacchi-Pilone, G., Mahon, K. & Gall, J. Cell 24, 649–659 (1981).

    Article  CAS  Google Scholar 

  34. Yunis, J. Hum. Genet. 56, 293–298 (1981).

    Article  CAS  Google Scholar 

  35. Yunis, J., Sawyer, J. & Ball, D. Chromosoma 67, 293–307 (1978).

    Article  CAS  Google Scholar 

  36. Renwick, J. Br. med. Bull. 25, 65–73 (1969).

    Article  CAS  Google Scholar 

  37. Goss, S. J. & Harris, H. Nature 255, 680–684 (1975).

    Article  ADS  CAS  Google Scholar 

  38. Goss, S. Cytogenet. Cell Genet. 25, 1–4 (1979).

    Article  Google Scholar 

  39. Ruddle, F. H. & McBride, W. in Molecular Biology of the Mammalian Genetic Apparatus (Elsevier, Amsterdam, 1977).

    Google Scholar 

  40. Klobutcher, L. A. & Ruddle. F. H. Nature 280, 657–660 (1979).

    Article  ADS  CAS  Google Scholar 

  41. Wilson, E. B. Arch. Mikrosk. Anat. 77, 249–271 (1911).

    Article  Google Scholar 

  42. Levine, P. & Stetson, R. E. J. Am. med. Ass. 113, 126–127 (1939).

    Article  Google Scholar 

  43. Van Cong, N., Billardon, C., Picard, J., Feingold, J. & Frézal, J. Cr. hebd. Séanc. Acad. Sci., Paris 272, 485 (1971).

    Google Scholar 

  44. Ruddle, F. H. et al. Science 176, 1429–1431 (1972).

    Article  ADS  CAS  Google Scholar 

  45. McKusick, V. A. in Mendelian Inheritance in Man (Johns Hopkins University Press, Baltimore, 1978).

    Google Scholar 

  46. Botstein, D., White, R., Skolnick, M. & Davis, R. Am. J. hum. Genet. 32, 314–331 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  47. Wyman, A. & White, R. Proc. natn. Acad. Sci. U.S.A. 77, 6754–6758 (1980).

    Article  ADS  CAS  Google Scholar 

  48. DeMartinville, B., Wyman, A., White, R. & Francke, U. Pediat. Res. 15, 560 (1981).

    Article  Google Scholar 

  49. Woods, D., Crampton, J., Clarke, B. & Williamson, R. Nucleic Acids Res. 8, 5157–5168 (1980).

    Article  CAS  Google Scholar 

  50. Gusella, J. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2829–2833 (1980).

    Article  ADS  CAS  Google Scholar 

  51. Kan, Y. & Dozy, A. Proc. natn. Acad. Sci. U.S.A. 75, 5631–5635 (1978).

    Article  ADS  CAS  Google Scholar 

  52. Phillips, J. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2853–2856 (1980).

    Article  ADS  CAS  Google Scholar 

  53. Geever, R. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  54. Owerbach, D. et al. Somatic Cell Genet. 7, 359–369 (1981).

    Article  CAS  Google Scholar 

  55. Owerbach, D., Rutter, W., Cooke, N., Martial, J. & Shows, T. Science 212, 815–816 (1981).

    Article  ADS  CAS  Google Scholar 

  56. Owerbach, D. et al. Proc. natn. Acad. Sci. U.S.A. 78, 3123–3127 (1981).

    Article  ADS  CAS  Google Scholar 

  57. Owerbach, D., Bell, G., Rutter, W. & Shows, T. Nature 286, 82–84 (1980).

    Article  ADS  CAS  Google Scholar 

  58. Harper, M. & Sanders, G. Eur. J. Cell Biol. 22, 20 (1980).

    Google Scholar 

  59. Owerbach, D., Rutter, W., Martial, J., Baxter, J. & Shows, T. Science 209, 289–292 (1980).

    Article  ADS  CAS  Google Scholar 

  60. George, D., Phillips, J., Francke, U. & Seeburg, P. Hum. Genet. 57, 138–141 (1981).

    Article  CAS  Google Scholar 

  61. Wolf, S., Mareni, C. & Migeon, B. Cell 21, 95–102 (1980).

    Article  CAS  Google Scholar 

  62. D'Eustachio, P., Meyuhas, O., Ruddle, F. H. & Perry, R. P. Cell 24, 307–312 (1981).

    Article  CAS  Google Scholar 

  63. Yagi, M., D'Eustachio, P., Ruddle, F. H. & Koshland, M. J. exp Med. (in the press).

  64. Roberts, M., Huttner, K. M., Schimke, R. T. & Ruddle, F. H. J. Cell Biol. 87, SG2211 (1980).

  65. D'Eustachio, P., Ingram, P. S., Tilghman, S. M. & Ruddle, F. H. Somatic Cell Genet. 7, 289–294 (1981).

    Article  CAS  Google Scholar 

  66. Leder, A., Swan, D., Ruddle, F., D'Eustachio, P. & Leder, P. Nature 293, 196–200 (1981).

    Article  ADS  CAS  Google Scholar 

  67. Gupta, P., D'Eustachio, P., Ruddle, F. & Rosen, J. J. Cell Biol. 87, D841 (1980).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Yale University, PO Box 6666, New Haven, Connecticut, 06511, USA

    F. H. Ruddle

Authors
  1. F. H. Ruddle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ruddle, F. A new era in mammalian gene mapping: somatic cell genetics and recombinant DNA methodologies. Nature 294, 115–120 (1981). https://doi.org/10.1038/294115a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/294115a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing