Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Extracellular phospholipase A2 mediates inflammatory hyperaemia

Nature volume 293, pages 583–585 (1981)Cite this article

Abstract

Inflammatory erythema is primarily the gross manifestation of enhanced regional blood flow or hyperaemia. Hyperaemia to areas of injury is thought to modulate the delivery of circulating inflammatory cells to the responding site1,2 and in experimental models, it was shown synergistically to enhance the extravasation of plasma proteins and mediators3,4. It has recently been proposed that blood flow to inflammatory sites may be modulated by a novel vasoactive moiety, distinct from prostaglandins and plasminogen activator5,6, which is released by macrophages in response to antigenic or mitogenic stimulation. Intradermal (i.d.) injection of this hyperaemia-inducing activity (HIA) from activated macrophages elicited a sustained hyperaemia in the test rabbit7. We have now investigated this vasoactive mediator further. Using a sensitive assay for phospholipase A2 (PLA2)8, we have identified this enzyme in vasoactive conditioned medium of glycogen-induced peritoneal exudate cells (PEC) of rabbits. In the vasoactive conditioned medium, PLA2 and HIA cofractionated on gel filtration chromatography, and both the enzyme activity and biological activity were abrogated by treatment with the active site-directed histidine reagent p-bromophenacyl bromide (pBPB). Intradermal injection of PLA2 purified from snake venom induced a hyperaemia with kinetics resembling those of HIA. We propose that antigen-induced release of PLA2 by activated macrophages may mediate the hyperaemia associated with sites of chronic inflammation.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hay, J. B. & Hobbs, B. B. J. exp. Med. 145, 31–44 (1977).

    Article  CAS  Google Scholar 

  2. Ottaway, C. A. & Parrott, D. M. V. J. exp. Med. 150, 218–230 (1979).

    Article  CAS  Google Scholar 

  3. Johnston, M. G., Hay, J. B. & Movat, H. Z. Agents & Actions 6, 705–711 (1976).

    Article  CAS  Google Scholar 

  4. Williams, T. J. & Peck, M. J. Nature 270, 530–532 (1977).

    Article  ADS  CAS  Google Scholar 

  5. Vadas, P., Wasi, S., Movat, H. Z. & Hay, J. B. Proc. exp. Biol. Med. 161, 82–85 (1979).

    Article  CAS  Google Scholar 

  6. Vadas, P. & Hay, J. B. Int. Archs Allergy appl. Immun. 62, 142 (1980).

    Article  CAS  Google Scholar 

  7. Vadas, P. & Hay, J. B. Agents & Actions 8, 504–508 (1978).

    Article  CAS  Google Scholar 

  8. Vadas, P. & Hay, J. B. Life Sci. 26, 1721–1729 (1980).

    Article  CAS  Google Scholar 

  9. Patriarca, P., Beckerdite, S. & Elsbach, P. Biochim. biophys. Acta 260, 593–600 (1972).

    Article  CAS  Google Scholar 

  10. Roberts, M. F., Deems, R. A., Mincey, T. C. & Dennis, E. A. J. biol. Chem. 252, 2405–2411 (1977).

    CAS  PubMed  Google Scholar 

  11. Evenberg, A., Meyer, H., Verheij, H. M. & DeHaas, G. H. Biochim. biophys. Acta 491, 265–274 (1977).

    Article  CAS  Google Scholar 

  12. Arenti, G., Leat, W. M. F. & Harrison, F. A. Q. J. exp. Physiol. 60, 15–24 (1975).

    Article  Google Scholar 

  13. DeHaas, G. H., Postema, N. M., Nieuwenhuizen, W. & Van Deenen, L. L. M. Biochim. biophys. Acta 159, 103–117 (1968).

    Article  CAS  Google Scholar 

  14. Shaw, J. O., Roberts, M. F., Ulevitch, R. J., Henson, P. & Dennis, E. A. Am. J. Path. 91, 517–530 (1978).

    CAS  PubMed  Google Scholar 

  15. Okada, H. & Cyong, J-C. Jap. J. exp. Med. 45, 533–534 (1975).

    CAS  PubMed  Google Scholar 

  16. Phillips, G. B., Bachner, P. & McKay, D. G. Proc. Soc. exp. Biol. Med. 119, 846–850 (1965).

    Article  CAS  Google Scholar 

  17. Gerrard, J. M. et al. Am. J. Path. 96, 423–436 (1979).

    CAS  Google Scholar 

  18. Tokumura, A., Fukuzawa, K. & Tsukatani, H. Lipids 13, 572–574 (1978).

    Article  CAS  Google Scholar 

  19. Vallee, E., Gougat, J., Navarro, J. & Delahayes, J. F. J. Pharm. Pharmac. 31, 588–592 (1979).

    Article  CAS  Google Scholar 

  20. Franson, R., Dobrow, R., Weiss, J., Elsbach, P. & Weglicki, W. B. J. Lipid Res. 19, 18–23 (1978).

    CAS  PubMed  Google Scholar 

  21. Vadas, P. & Hay, J. B. Am. J. Path. (submitted).

  22. Dawson, R. M. C. & Irvine, R. F. Advances in Prostaglandin and Thromboxane Research (eds Galli, C. et al.) (Raven, New York, 1978).

    Google Scholar 

  23. Danon, A. & Assouline, G. Nature 273, 552–554 (1978).

    Article  ADS  CAS  Google Scholar 

  24. Flower, R. J. & Blackwell, G. J. Nature 278, 456–459 (1979).

    Article  ADS  CAS  Google Scholar 

  25. Flower, R. Advances in Prostaglandin and Thromboxane Research Vol. 3. (eds Galli, C. et al.) (Raven, New York, 1978).

    Google Scholar 

  26. Vargaftig, B. B. & Dao Hai, J. J. Pharm. Pharmac. 24, 159–161 (1972).

    Article  CAS  Google Scholar 

  27. Vadas, P. Life Sci. (in the press).

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Medical Sciences Building, University of Toronto, Toronto, Canada, M5S 1A8

    Peter Vadas, Safia Wasi, Henry Z. Movat & John B. Hay

Authors
  1. Peter Vadas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Safia Wasi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Henry Z. Movat
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John B. Hay
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vadas, P., Wasi, S., Movat, H. et al. Extracellular phospholipase A2 mediates inflammatory hyperaemia. Nature 293, 583–585 (1981). https://doi.org/10.1038/293583a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/293583a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing