Abstract
The bacteriophage λ regulatory protein, cII, has been purified and shown to activate positively RNA transcription from the two phage promoters which coordinately regulate phage lysogenic development. To obtain this protein, the cII gene was cloned into a plasmid vector carrying the strong, regulatable λ phage promoter PL such that it was overproduced to levels approaching 5% of cellular protein.
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Shimatake, H., Rosenberg, M. Purified λ regulatory protein cII positively activates promoters for lysogenic development. Nature 292, 128–132 (1981). https://doi.org/10.1038/292128a0
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DOI: https://doi.org/10.1038/292128a0
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