Abstract
DNA-dependent RNA polymerase II purified from healthy plant tissue is capable of synthesizing linear (−)-viroid RNA copies of full length from (+)-viroid RNA templates in vitro. Together with the specific α-amanitin sensitivity of viroid replication observed in vivo, these findings suggest that viroids replicate by an entirely novel mechanism in which infecting viroid RNA molecules are copied by the host enzyme which is normally responsible for the synthesis of nuclear precursors to messenger RNA.
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References
Sänger, H. L., Klotz, G., Riesner, D., Gross, H. J. & Kleinschmidt, A. K. Proc. natn. Acad. Sci. U.S.A. 73, 3852–3856 (1976).
Gross, H. J. et al. Nature 273, 203–208 (1978).
Riesner, D. J. molec. Biol. 133, 85–115 (1979).
Semancik, J. S. & Geelen, J. L. M. C. Nature 256, 753–756 (1975).
Hadidi, A., Jones, D. M., Gillespie, D. H., Wong-Staal, F. & Diener, T. O. Proc. natn. Acad. Sci. U.S.A. 73, 2453–2457 (1976).
Diener, T. O. & Hadidi, A. Comprehensive Virol. 11, 285–335 (1977).
Zaitlin, M., Niblett, C. L., Dickson, E. & Goldberg, R. B. Virology 104, 1–9 (1980).
Branch, A. D. & Dickson, E. Virology 104, 10–26 (1980).
Mühlbach, H.-P. & Sänger, H. L. Nature 278, 185–188 (1979).
Jendrisak, J. J. & Burgess, R. R. Biochemistry 14, 4639–4645 (1975).
Sasaki, R., Goto, H., Arima, K. & Sasaki, Y. Biochim. biophys. Acta 366, 435–442 (1974).
Sasaki, Y., Goto, H., Wake, T. & Sasaki, R. Biochim. biophys. Acta 366, 443–453 (1974).
Sasaki, Y., Goto, H., Ohta, H. & Kamikubo, T. Eur. J. Biochem. 70, 369–375 (1976).
Gross, H. J., Domdey, H. & Sänger, H. L. Nucleic Acids Res. 4, 2021–2028 (1977).
Sänger, H. L. et al. FEBS Lett. 99, 117–122 (1979).
Wieland, Th. & Faulstich, H. Crit. Rev. Biochem. 5, 185–260 (1978).
Weinman, R. & Roeder, R. G. Proc. natn. Acad. Sci. U.S.A. 71, 1790–1794 (1974).
Boege, F. & Sänger, H. L. FEBS Lett. 121, 91–96 (1980).
Seidman, S., Surzycki, S. J., De Lorbe, W. & Gussin, G. N. Biochemistry 18, 3363–3371 (1979).
Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).
Grill, L. K. & Semancik, J. S. Proc. natn. Acad. Sci. U.S.A. 75, 896–900 (1978).
Grill, L. K., Negruk, V. I. & Semancik, J. S. Virology 107, 24–33 (1980).
Mills, D. R., Dobkin, C. & Kramer, F. R. Cell 15, 541–550 (1978).
Diener, T. O. Virology 43, 75–89 (1971).
Sänger, H. L. Adv. Biosci. 8, 103–116 (1972).
Takahashi, T. & Diener, T. O. Virology 64, 106–114 (1975).
Mühlbach, H.-P. & Sänger, H. L. Biosci. Rep. (in the press).
Rackwitz, H. R., Mühlbach, H.-P. & Sänger, H. L. Hoppe Seyler's Z. physiol. Chem. 361, 316 (1980).
Commerford, S. L. Biochemistry 10, 1993–1999 (1971).
Rohde, W. & Sänger, H. L. Biosci. Rep. 1, No. 4 (in the press).
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Rackwitz, HR., Rohde, W. & Sänger, H. DNA-dependent RNA polymerase II of plant origin transcribes viroid RNA into full-length copies. Nature 291, 297–301 (1981). https://doi.org/10.1038/291297a0
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DOI: https://doi.org/10.1038/291297a0
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