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Polyoma DNA sequences involved in control of viral gene expression in murine embryonal carcinoma cells

Nature volume 290, pages 720–722 (1981)Cite this article

Abstract

Polyoma virus can develop lytically in differentiated mouse cells, but is unable to express either early or late functions in undifferentiated early embryonic cells1 or in embryonal carcinoma (EC) cells of the mouse. However, the EC cells become susceptible during differentiation and then behave as do mouse somatic cells2. We have recently described the isolation of a new class of polyoma virus mutant (Py EC) able to develop normally in an EC cell line (PCC4-Aza) that is capable of trigerminal differentiation in vivo3 but exhibits a very limited differentiation in vitro4. The mutants do not grow in another EC cell line (F9) that is ‘blocked’—‘nullipotent’ in vivo, but able to differentiate in vitro when grown in the presence of chemical inducers. Compared with their parental strain, all Py EC genomes analysed so far have an extra 20–50 bases in their HpaII-3 restriction fragment5. We now describe polyoma mutants that will grow in EC F9 cells, and the sequence changes in two of them. The region involved in the mutations can be folded into a putative tRNA-like secondary structure which may be involved in the regulation of viral early transcription with respect to the state of differentiation of the host cell.

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References

  1. Swartzendruber, D. E. & Lehman, J. M. J. cell. Physiol. 85, 179–187 (1975).

    Article  CAS  Google Scholar 

  2. Boccara, M. & Kelly, F. Annls Microbiol. Inst. Pasteur, Paris A129, 227–238 (1978).

    CAS  Google Scholar 

  3. Jakob, H., Boon, T., Gaillard, J., Nicolas, J. F. & Jacob, F. Annls Microbiol. Inst. Pasteur Paris B124, 269–282 (1973).

    CAS  Google Scholar 

  4. Shermann, M. L. in Teratomas and Differentiation (eds Shermann, M. C. & Solter, D.) 189–205 (Academic, New York, 1975).

    Google Scholar 

  5. Soeda, E., Arrand, J. R., Smolar, N., Walsh, J. E. & Griffin, B. E. Nature 283, 445–453 (1980).

    Article  ADS  CAS  Google Scholar 

  6. Seif, R. & Cuzin, F. J. Virol. 24, 721–728 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Strickland, S. & Mahdavi, V. Cell 15, 393–403 (1978).

    Article  CAS  Google Scholar 

  8. Pope, J. M. & Rowe, W. P. J. exp. Med. 120, 121–128 (1964).

    Article  CAS  Google Scholar 

  9. Vasseur, M., Kress, C., Montreau, N. & Blangy, D. Proc. natn. Acad. Sci. U.S.A. 77, 1068–1072 (1980).

    Article  ADS  CAS  Google Scholar 

  10. Arrand, J. R. et al. J. Virol. 33, 606–618 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Magnusson, G. & Nilson, M. G. J. Virol. 22, 646–653 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Katinka, M., Yaniv, M., Vasseur, M. & Blangy, D. Cell 20, 393–399 (1980).

    Article  CAS  Google Scholar 

  13. Segal, S., Levine, A. J. & Khoury, G. Nature 280, 335–337 (1979).

    Article  ADS  CAS  Google Scholar 

  14. Inokuchi, H., Dove, W. F. & Freifelder, D. J. molec. Biol. 74, 721–727 (1973).

    Article  CAS  Google Scholar 

  15. Liu, L. F., Liu, C. C. & Alberts, B. M. Cell 19, 697–707 (1980).

    Article  CAS  Google Scholar 

  16. Hseih, T. & Brutlag, D. Cell 21, 115–125 (1980).

    Article  Google Scholar 

  17. Grosschedl, R. & Birnstiel, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 1432–1436 (1980).

    Article  ADS  CAS  Google Scholar 

  18. Benoist, C. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 77, 3865–3869 (1980).

    Article  ADS  CAS  Google Scholar 

  19. Dandolo, L. et al. (in preparation).

  20. Berkner, K. L. & Folk, W. R. J. biol. Chem. 252, 3176–3184 (1977).

    CAS  Google Scholar 

  21. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560.

  22. Sanger, F. & Coulson, A. FEBS Lett. 87, 107–110 (1978).

    Article  CAS  Google Scholar 

  23. Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–318 (1977).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Département de Biologie moléculaire, Institut Pasteur, 25 rue du Dr Roux, 75015, Paris, France

    Michaël Katinka & Moshe Yaniv

  2. Laboratoire des Virus oncogénes, Université Pierre et Marie Curie, Institut de Recherches Scientifiques sur le Cancer, BP no 8, 94800, Villejuif, France

    Marc Vasseurt, Nicole Montreau & Daniel Blangy

Authors
  1. Michaël Katinka
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  3. Nicole Montreau
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  4. Moshe Yaniv
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  5. Daniel Blangy
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Katinka, M., Vasseurt, M., Montreau, N. et al. Polyoma DNA sequences involved in control of viral gene expression in murine embryonal carcinoma cells. Nature 290, 720–722 (1981). https://doi.org/10.1038/290720a0

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