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Central noradrenergic neurones concentrate 3H-oestradiol

Nature volume 289, pages 500–502 (1981)Cite this article

Abstract

Central catecholamines play an important part in the regulation of hormone secretion from the pituitary gland and in the mediation of male and female sexual behaviour1–6. Noradrenaline has been shown to stimulate the release of luteinizing hormone (LH) probably by influencing the secretion of LH-releasing hormone (LHRH)7,8. The medial preoptic and hypothalamic areas, including the median eminence, contain noradrenaline-containing terminals which originate from discrete noradrenaline-containing cell groups in the lower brain stem. These cell groups have been identified in the pons and the medulla oblongata by histochemical methods and pharmacological experiments9–14. Recent studies with antiserum to dopamine-β-hydroxylase (DBH), the enzyme that converts dopamine to noradrenaline, provided evidence for the existence of noradrenergic cells15,16. These include the locus coeruleus (group A6), a ventrally located more diffuse but continuous subcoeruleus group (group A5), a cell group located dorsal to the nucleus (n.) dorsalis motorius nervi vagi (group A2) and a cell group in or near the nucleus reticularis lateralis (group A1). Using the thaw-mount autoradiographic technique, oestradiol-concentrating neurones have been localized in many areas of the lower brain stem, including the locus coeruleus, n. tractus solitarii, n. dorsalis motorius nervi vagi, and reticular formation17 where catecholamine-containing neurones exist. We report here the simultaneous localization, in the same histological section, of 3H-oestradiol and the enzyme dopamine-β-hydroxylase in neurones of the rat lower brain stem with a combined technique of thaw-mount autoradiography and immunohistochemistry18,19, demonstrating that noradrenaline-or adrenaline-containing neurones are oestradiol target cells.

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References

  1. Kalra, P. S., Kalra, S. P., Krulich, L., Fawcett, C. P. & McCann, S. M. Endocrinology 90, 1168–1176 (1972).

    Article  CAS  Google Scholar 

  2. Kalra, S. P. & McCann, S. M. Prog. Brain Res. 39, 185 (1973).

    Article  CAS  Google Scholar 

  3. Fuxe, K., Hökfelt, T. & Jonsson, G. J. Comp. Physiol. Psychol. 89, 556 (1975).

    Article  Google Scholar 

  4. Mahnnas, C. O. in Pharmacology, Biochemistry and Behavior Vol. 4, 521 (Ankho Intl. Inc., 1976).

    Google Scholar 

  5. McCann, S. M. & Moss, R. L. Life Sci. 16, 833 (1975).

    Article  CAS  Google Scholar 

  6. Herndon, J. G. Jr. Physiol. Behav. 17, 143 (1976).

    Article  Google Scholar 

  7. Simpkins, J. W. & Kalra, S. P. Brain Res. 170, 475 (1979).

    Article  CAS  Google Scholar 

  8. Honma, K. & Wuttke, W. Endocrinology 106, 1848 (1980).

    Article  CAS  Google Scholar 

  9. Dahlström, A. & Fuxe, K. Acta physiol. scand. 62, Suppl. 232, 1, 55 (1964).

    Google Scholar 

  10. Anden, N. E. et al. Acta physiol. scand. 67, 313 (1966).

    Article  CAS  Google Scholar 

  11. Fuxe, K. & Dahlström, A. Pharmac. Rev. 18, 727 (1966).

    Google Scholar 

  12. Corrodi, H., Fuxe, K., Hamberger, B. & Liungdahl, A. Eur. J. Pharmac. 12, 145 (1970).

    Article  CAS  Google Scholar 

  13. Palkovits, M. & Jacobowitz, D. M. J. comp. neurol. 157, 29 (1974).

    Article  CAS  Google Scholar 

  14. Moore, R. Y. & Bloom, F. E. A. Rev. Neurosci. 2, 113 (1979).

    Article  CAS  Google Scholar 

  15. Fuxe, K., Goldstein, M., Hökfelt, T. & Joh, T. H. Res. Commun. chem. Path. Pharmac. 1, 627 (1970).

    CAS  Google Scholar 

  16. Swanson, L. W. & Hartman, B. K. J. comp. Neurol. 163, 467 (1975).

    Article  CAS  Google Scholar 

  17. Stumpf, W. E. & Sar, M. in Anatomical Neuroendocrinology (eds Stumpf, W. E. & Grant, L. D.) 254 (Karger, Basel, 1975).

    Google Scholar 

  18. Sar, M. & Stumpf, W. E. Cell Tissue Res. 203, l (1979).

    Article  Google Scholar 

  19. Sar, M. & Stumpf, W. E. J. Histochem. Cytochem. (in the press).

  20. Stumpf, W. E. & Sar, M. Meth. Enzym. 36, 135 (1975).

    Article  CAS  Google Scholar 

  21. Straus, W. J. Histochem. Cytochem. 27, 1349 (1979).

    Article  CAS  Google Scholar 

  22. Pickel, V. M., Joh, T. H. & Reis, D. J. J. Histochem. Cytochem. 24, 792 (1976).

    Article  CAS  Google Scholar 

  23. Stumpf, W. E. & Sar, M. in Modem Pharmacology–Toxicology Vol. 8 (ed. Pasqualini, J.) 41 (Dekker, New York, 1976).

    Google Scholar 

  24. Heritage, A. S., Grant, L. D. & Stumpf, W. E. J. comp. Neurol. 176, 607 (1977).

    Article  CAS  Google Scholar 

  25. Hökfelt, J., Fuxe, K., Goldstein, M. & Johnson, O. Brain Res. 66, 235 (1974).

    Article  Google Scholar 

  26. Stefane, F. J. E., Donoso, A. O. & Curier, J. Acta physiol. latinoam. 15, 425 (1965).

    Google Scholar 

  27. Donoso, A. O. & Stafano, F. J. E. Experientia 23, 665 (1967).

    Article  CAS  Google Scholar 

  28. Crowley, W. R., O'Donohue, T. L., Wachslietht, H. & Jacobowitz, D. M. Brain Res. 154, 345 (1978).

    Article  CAS  Google Scholar 

  29. Beattie, C. W., Rodgers, C. H. & Soyka, L. F. Endocrinology 91, 276 (1972).

    Article  CAS  Google Scholar 

  30. Luine, V. N. & McEwen, B. S. J. Neurochem. 28, 1221 (1977).

    Article  CAS  Google Scholar 

  31. Nicholson, G., Greely, G., Humo, J., Youngblood, W. & Kizer, J. S. Endocrinology 103, 559 (1978).

    Article  CAS  Google Scholar 

  32. Martinovic, J. & McCann, S. Endocrinology 100, 1206 (1977).

    Article  CAS  Google Scholar 

  33. Negro-Vilar, A., Ojeda, S. R., Davis, J. P. & McCann, S. Endocrinology 105, 86 (1979).

    Article  CAS  Google Scholar 

  34. Simpkins, J. W., Huang, H. H., Davis, J. P. & Meites, J. Biol. Reprod. 20, 625 (1979).

    Article  CAS  Google Scholar 

  35. Kalra, S. P., Kalra, P. S., Chen, C. L. & Clemens, J. A. Acta endocr. 89, 1 (1978).

    Article  CAS  Google Scholar 

  36. Kun, L. Rev. Rom. med. Endocr. 16, 263 (1978).

    CAS  Google Scholar 

  37. Day, T. A., Blessing, W. & Willoughby, J. O. Brain Res. 193, 543 (1980).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Departments of Anatomy and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, 27514

    Madhabananda Sar & Walter E. Stumpf

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  1. Madhabananda Sar
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  2. Walter E. Stumpf
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Sar, M., Stumpf, W. Central noradrenergic neurones concentrate 3H-oestradiol. Nature 289, 500–502 (1981). https://doi.org/10.1038/289500a0

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