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Actin-containing matrix associated with the plasma membrane of murine tumour and lymphoid cells

Abstract

A detergent-insoluble matrix has been isolated from murine tumour cell and lymphocyte plasma membranes. The major components of this matrix include actin and four additional proteins not previously identified as cytoskeletal proteins. Labelling studies indicate that the matrix is located on the inner face of the plasma membrane. A cell-surface glycoprotein, 5′-nucleotidase, remains associated with the isolated matrix.

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References

  1. Bretscher, M. S. & Raff, M. C. Nature 258, 43–49 (1975).

    Article  ADS  CAS  Google Scholar 

  2. LaCelle, P. L. & Kirkpatrick, F. H. in Erythrocyte Structure and Function (ed. Brewer, G. J.) 535–557 (Liss, New York, 1975).

    Google Scholar 

  3. Sheetz, M. P. & Singer, S. J. J. Cell Biol. 73, 638–646 (1977).

    Article  CAS  Google Scholar 

  4. Nicolson, G. L. & Painter, R. G. J. Cell Biol. 59, 395–406 (1973).

    Article  CAS  Google Scholar 

  5. Elgsaeter, A. & Branton, D. J. Cell Biol. 63, 1018–1036 (1974).

    Article  CAS  Google Scholar 

  6. Yu, J., Fischman, D. A. & Steck, T. L. J. supramolec. Struct. 1, 233–248 (1973).

    Article  CAS  Google Scholar 

  7. Lux, S. E. Nature 281, 426–429 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Nicolson, G. L. Biochim. biophys. Acta 457, 57–108 (1976).

    Article  CAS  Google Scholar 

  9. Lazarides, E. Nature 283, 249–256 (1980).

    Article  ADS  CAS  Google Scholar 

  10. Edelman, G. M. Science 192, 218–226 (1976).

    Article  ADS  CAS  Google Scholar 

  11. dePetris, S. & Raff, M. C. Eur. J. Immun. 2, 523–535 (1972).

    Article  CAS  Google Scholar 

  12. Schreiner, G. F., Fujiwara, K., Pollard, T. D. & Unanue, E. R. J. exp. Med. 145, 1393–1398 (1977).

    Article  CAS  Google Scholar 

  13. Korn, E. D. in Cold Spring Harb. Conf. Cell Proliferation Vol. 13 (eds Goldman, P., Pollard, T. & Rosenbaum, J.) 623–630 (Cold Spring Harbor Laboratory, New York, 1967).

    Google Scholar 

  14. Rees, D. A., Lloyd, C. W. & Thom, D. Nature 267, 1240128 (1977).

  15. Koch, G. & Smith, M. Nature 273, 274–278 (1978).

    Article  ADS  CAS  Google Scholar 

  16. Flanagan, J. & Koch, G. Nature 273, 278–281 (1978).

    Article  ADS  CAS  Google Scholar 

  17. Carter, W. R. & Hakomori, S. J. biol. Chem. 253, 2867–2874 (1978).

    CAS  PubMed  Google Scholar 

  18. Lemonnier, F., Mescher, M., Sherman, L. & Burakoff, S. J. Immun. 120, 1114–1120 (1978).

    CAS  PubMed  Google Scholar 

  19. Crumpton, M. J. & Snary, D. Contemp. Topics molec. Immun. 3, 27–56 (1974).

    Article  CAS  Google Scholar 

  20. Pollard, T. D. & Weihing, R. R. CRC crit. Rev. Biochem. 2, 1–65 (1964).

    Article  Google Scholar 

  21. Barber, B. H. & Crumpton, M. J. FEBS Lett. 66, 215–220 (1976).

    Article  CAS  Google Scholar 

  22. Cleveland, D. W., Fischer, S. G., Kirschner, M. W. & Laemmli, U. K. J. biol. Chem. 252, 1102–1106 (1977).

    CAS  Google Scholar 

  23. Bourguignon, L. Y. W. & Singer, S. J. Proc. natn. Acad. Sci. U.S.A. 74, 5031–5035 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Loor, F. Nature 264, 272–273 (1976).

    Article  ADS  CAS  Google Scholar 

  25. Hoessli, D., Rungger-Brändle, E., Jockusch, B. M. & Gabbiani, G. J. Cell Biol. 84, 305–314 (1980).

    Article  CAS  Google Scholar 

  26. Misra, D. N., Gill, T. J. III & Estes, L. W. Biochim. biophys. Acta 352, 455–461 (1974).

    CAS  PubMed  Google Scholar 

  27. Uusitalo, R. J. & Karnovsky, J. J. J. Histochem. Cytochem. 25, 87–96 (1976).

    Article  Google Scholar 

  28. Dornand, J., Bonnafous, J.-C. & Maini, J.-C. Eur. J. Biochem. 87, 459–465 (1978).

    Article  CAS  Google Scholar 

  29. Herrmann, S. H. & Mescher, M. F. Proc. natn. Acad. Sci. U.S.A. (submitted).

  30. Carraway, K. L., Doss, R. C., Huggins, J. W., Chesnut, R. W. & Carraway, C. A. C. J. Cell Biol. 83, 529–543 (1979).

    Article  CAS  Google Scholar 

  31. Gruenstein, E., Rich, A. & Weihing, R. R. J. Cell Biol. 64, 223–234 (1975).

    Article  CAS  Google Scholar 

  32. Moore, P. B., Ownby, C. L. & Carraway, K. L. Expl Cell Res. 115, 331–342 (1978).

    Article  CAS  Google Scholar 

  33. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).

    CAS  Google Scholar 

  34. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  35. Mescher, M. F. & Pollock, R. R. J. Immun. 123, 1155–1161 (1979).

    CAS  PubMed  Google Scholar 

  36. Avruch, J. & Wallach, D. F. H. Biochim. biophys. Acta 233, 334–347 (1971).

    Article  CAS  Google Scholar 

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Mescher, M., Jose, M. & Balk, S. Actin-containing matrix associated with the plasma membrane of murine tumour and lymphoid cells. Nature 289, 139–144 (1981). https://doi.org/10.1038/289139a0

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