Abstract
A detergent-insoluble matrix has been isolated from murine tumour cell and lymphocyte plasma membranes. The major components of this matrix include actin and four additional proteins not previously identified as cytoskeletal proteins. Labelling studies indicate that the matrix is located on the inner face of the plasma membrane. A cell-surface glycoprotein, 5′-nucleotidase, remains associated with the isolated matrix.
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References
Bretscher, M. S. & Raff, M. C. Nature 258, 43–49 (1975).
LaCelle, P. L. & Kirkpatrick, F. H. in Erythrocyte Structure and Function (ed. Brewer, G. J.) 535–557 (Liss, New York, 1975).
Sheetz, M. P. & Singer, S. J. J. Cell Biol. 73, 638–646 (1977).
Nicolson, G. L. & Painter, R. G. J. Cell Biol. 59, 395–406 (1973).
Elgsaeter, A. & Branton, D. J. Cell Biol. 63, 1018–1036 (1974).
Yu, J., Fischman, D. A. & Steck, T. L. J. supramolec. Struct. 1, 233–248 (1973).
Lux, S. E. Nature 281, 426–429 (1979).
Nicolson, G. L. Biochim. biophys. Acta 457, 57–108 (1976).
Lazarides, E. Nature 283, 249–256 (1980).
Edelman, G. M. Science 192, 218–226 (1976).
dePetris, S. & Raff, M. C. Eur. J. Immun. 2, 523–535 (1972).
Schreiner, G. F., Fujiwara, K., Pollard, T. D. & Unanue, E. R. J. exp. Med. 145, 1393–1398 (1977).
Korn, E. D. in Cold Spring Harb. Conf. Cell Proliferation Vol. 13 (eds Goldman, P., Pollard, T. & Rosenbaum, J.) 623–630 (Cold Spring Harbor Laboratory, New York, 1967).
Rees, D. A., Lloyd, C. W. & Thom, D. Nature 267, 1240128 (1977).
Koch, G. & Smith, M. Nature 273, 274–278 (1978).
Flanagan, J. & Koch, G. Nature 273, 278–281 (1978).
Carter, W. R. & Hakomori, S. J. biol. Chem. 253, 2867–2874 (1978).
Lemonnier, F., Mescher, M., Sherman, L. & Burakoff, S. J. Immun. 120, 1114–1120 (1978).
Crumpton, M. J. & Snary, D. Contemp. Topics molec. Immun. 3, 27–56 (1974).
Pollard, T. D. & Weihing, R. R. CRC crit. Rev. Biochem. 2, 1–65 (1964).
Barber, B. H. & Crumpton, M. J. FEBS Lett. 66, 215–220 (1976).
Cleveland, D. W., Fischer, S. G., Kirschner, M. W. & Laemmli, U. K. J. biol. Chem. 252, 1102–1106 (1977).
Bourguignon, L. Y. W. & Singer, S. J. Proc. natn. Acad. Sci. U.S.A. 74, 5031–5035 (1977).
Loor, F. Nature 264, 272–273 (1976).
Hoessli, D., Rungger-Brändle, E., Jockusch, B. M. & Gabbiani, G. J. Cell Biol. 84, 305–314 (1980).
Misra, D. N., Gill, T. J. III & Estes, L. W. Biochim. biophys. Acta 352, 455–461 (1974).
Uusitalo, R. J. & Karnovsky, J. J. J. Histochem. Cytochem. 25, 87–96 (1976).
Dornand, J., Bonnafous, J.-C. & Maini, J.-C. Eur. J. Biochem. 87, 459–465 (1978).
Herrmann, S. H. & Mescher, M. F. Proc. natn. Acad. Sci. U.S.A. (submitted).
Carraway, K. L., Doss, R. C., Huggins, J. W., Chesnut, R. W. & Carraway, C. A. C. J. Cell Biol. 83, 529–543 (1979).
Gruenstein, E., Rich, A. & Weihing, R. R. J. Cell Biol. 64, 223–234 (1975).
Moore, P. B., Ownby, C. L. & Carraway, K. L. Expl Cell Res. 115, 331–342 (1978).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).
Laemmli, U. K. Nature 227, 680–685 (1970).
Mescher, M. F. & Pollock, R. R. J. Immun. 123, 1155–1161 (1979).
Avruch, J. & Wallach, D. F. H. Biochim. biophys. Acta 233, 334–347 (1971).
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Mescher, M., Jose, M. & Balk, S. Actin-containing matrix associated with the plasma membrane of murine tumour and lymphoid cells. Nature 289, 139–144 (1981). https://doi.org/10.1038/289139a0
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DOI: https://doi.org/10.1038/289139a0
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