Abstract
We have examined the arrangement of the genes for the mouse heavy chain subclasses γ2b, γ2a and γ1 by the Southern hybridisation procedure. Evidence has been found for rearrangement involving the γ2b and γ2a CH genes in the DNA of cells making IgG2b andIgG2a respectively. The DNA of an IgG2b-secreting line lacks detectable Cγ1 gene, whilst the DNA of an IgG2a-secreting line lacks detectable Cγ1 and Cγ2b genes. The DNA of a cell line secreting IgA lacks detectable Cγ genes. These observations implicate deletion in the mechanism of the H-chain switch and allow a preliminary ordering of some of the CH genes in the mouse genome.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dreyer, W. J. & Bennett, J. C. Proc. natn. Acad. Sci. U.S.A. 54, 864–869 (1965).
Hozumi, N. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 73, 3628–3632 (1976).
Rabbitts, T. H. & Forster, A. Cell 13, 319–327 (1978).
Seidman, J. G. et al. Proc. natn. Acad. Sci. U.S.A. 575, 3881–3885 (1978).
Seidman, J. G. & Leder, P. Nature 276, 790–795 (1978).
Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).
Sakano, H., Hüppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).
Rabbitts, T. H. Nature 275, 291–296 (1978).
Gilmore-Hebert, M., Hercules, K., Komaromy, M. & Wall, R. Proc. natn. Acad. Sci. U.S.A. 75, 6044–6048 (1978).
Melchers, F., von Boehmer, H. & Phillips, R. A. Transplant Rev. 25, 26–58 (1975).
Cooper, M. D., Kearney, J. F., Lydyard, P. M., Grossi, C. E. & Lawton, A. R. Cold Spring Harb. Symp. quant. Biol. 41, 139–191 (1976).
Wang, A. C., Wilson, S. K., Hopper, J. E., Fudenberg, H. H. & Nisonoff, A. Proc. natn. Acad. Sci. U.S.A. 66, 337–343 (1970).
Sledge, C., Fair, D. S., Black, B., Krueger, R. G. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 73, 923–927 (1976).
Bevan, M. J., Parkhouse, R. M. E., Williamson, A. R. & Askonas, B. A. Prog. Biophys. 25, 131–162 (1972).
Tonegawa, S., Maxam, A. M., Tizard, R., Bernard, O. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 75, 1485–1489 (1978).
Honjo, T. & Kataoka, T. Proc. natn. Acad. Sci. U.S.A. 75, 2140–2144 (1978).
Schibler, U., Marcu, K. B. & Perry, R. P. Cell 15, 1495–1509 (1979).
Marcu, K. B., Schibler, U. & Perry, R. P. Science 204, 1087–1088 (1979).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Yamawaki-Kataoka, Y. et al. Biochemistry 18, 490–494 (1979).
Kataoka, T., Yamawaki-Kataoka, Y., Yamagishi, H. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 76, 4240–4244.
Sakano, H. et al. Nature 277, 627–633 (1979).
Landucci Tosi, S., Mage, R. G. & Dubiskis, S. J. Immun. 104, 641–647 (1970).
Kindt, T. J., Mandy, W. J. & Todd, C. W. Biochemistry 9, 2028–2032 (1970).
Herzenberg, L. A., McDevitt, H. O. & Herzenberg, L. A. A. Rev. Genet. 2, 209 (1968).
Brack, C., Hirama, M., Lenhard-Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).
Bernard, O., Hozumi, N. & Tonegawa, S. Cell 15, 1133–1144 (1978).
Weigert, M., Gatmaitan, L., Loh, E., Schilling, J. L. & Nature 276, 785–790 (1978).
Rao, N., Rudikoff, S., Krutzsch, H. & Potter, M. Proc. natn. Acad. Sci. U.S.A. 76, 2890–2894 (1979).
Early, P. W., Davis, M. M., Kabak, D. B., Davidson, W. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 76, 857–861 (1979).
Pernis, B., Forni, L. & Luzzati, A. L. Cold Spring Harb. Symp. quant. Biol. 41, 175–183 (1976).
Herzenberg, L. A. et al. Cold Spring Harb. Symp. quant. Biol. 41, 33 (1976).
Wall, R., Choi, E., Hercules, K., Komaromy, M. & Gilmore-Hebert, M. in Cells of Immunoglobulin Synthesis, Columbia University College of Physicians and Surgeons: Biomedical Sciences Symposium III 1978 (in the press).
Birshtein, B. K., Preud'homme, J.-L. & Scharff, M. D. Proc. natn. Acad. Sci. U.S.A. 71, 3478–3482 (1974).
Preud'homme, J.-L., Birshtein, B. K. & Scharff, M. D. Proc. natn. Acad. Sci. U.S.A. 72, 1427–1430 (1975).
Koskimies, S. & Birshstein, B. K. Nature 264, 480–482 (1976).
Liesegang, B., Radbruch, A. & Rajewsky, K. Proc. natn. Acad. Sci. U.S.A. 75, 3901–3905 (1978).
Denhardt, D. T. Biochem. biophys. Res. Commun. 23, 641–646 (1966).
Jeffreys, A. J. & Flavell, R. A. Cell 12, 429–439 (1977).
Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–316 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rabbitts, T., Forster, A., Dunnick, W. et al. The role of gene deletion in the immunoglobulin heavy chain switch. Nature 283, 351–356 (1980). https://doi.org/10.1038/283351a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/283351a0
This article is cited by
-
Linking class-switch recombination with somatic hypermutation
Nature Reviews Molecular Cell Biology (2001)
-
Comparative mapping ofIGHG, IGHM, FES, andFOS in domestic cattle
Immunogenetics (1992)
-
Immunoglobulin heavy-chain switching in pre-B leukaemias
Nature (1983)
-
ADP-ribosyl transferase, rearrangement of DNA, and cell differentiation
Bioscience Reports (1983)
-
Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing γ, ε and α genes
Nature (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.