Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

A UGA termination suppression tRNATrp active in rabbit reticulocytes

Nature volume 283, pages 41–46 (1980)Cite this article

Abstract

A tRNATrp was purified from rabbit reticulocytes which suppresses the UGA termination codon of β-haemoglobin mRNA. Evidence is presented that the β-haemoglobin readthrough protein is found in reticulocyte translations and intact cells. Some natural readthrough proteins perform essential functions; they are synthesised through suppression of UGA or UAG but not UAA termination codons.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Capecchi, M. R. & Klein, H. A. Cold Spring Harb. Symp. quant. Biol. 34, 469–477 (1969).

    Article  CAS  Google Scholar 

  2. Caskey, C. T., Scolnick, E., Tompkins, R., Goldstein, J. & Milman, G. Cold Spring. Harb. Symp. quant. Biol. 34, 479–489 (1969).

    Article  CAS  Google Scholar 

  3. Caskey, C. T., Beaudet, A. L. & Tate, W. P. Meth. Enzym. 30, 293–302 (1974).

    Article  CAS  Google Scholar 

  4. Korner, A. M., Feinstein, S. I. & Altman, S. in Transfer RNA (ed. Altman, S.) 105–135 (MIT Press, Cambridge, Massachusetts, 1979).

    Google Scholar 

  5. Horiuchi, K., Webster, R. E. & Sachiko, M. Virology 45, 429–439 (1971).

    Article  CAS  Google Scholar 

  6. Moore, C. H., Farron, F., Bohnert, D. & Weissman, C. Nature new Biol. 234, 204–206 (1971).

    Article  CAS  Google Scholar 

  7. Weiner, A. M. & Weber, K. Nature new Biol. 234, 206–209 (1971).

    Article  CAS  Google Scholar 

  8. Weiner, A. M. & Weber, K. J. molec. Biol. 80, 837–855 (1973).

    Article  CAS  Google Scholar 

  9. Hofstetter, H., Monstein, H. J. & Weissman, C. Biochim. biophys. Acta 374, 238–251 (1974).

    Article  CAS  Google Scholar 

  10. Yates, J. L., Gette, W. R., Furth, M. E. & Nomura, M. Proc. natn. Acad. Sci. U.S.A. 74, 689–693 (1977).

    Article  ADS  CAS  Google Scholar 

  11. Engelberg-Kulka, H., Dekel, L., Israeli-Reches, M. & Belfort, M. Molec. gen. Genet. 170, 155–159 (1979).

    Article  CAS  Google Scholar 

  12. Pelham, H. R. B. Nature 272, 469–471 (1978).

    Article  ADS  CAS  Google Scholar 

  13. Capecchi, M. R., Huges, S. H. & Wahl, G. M. Cell 6, 269–277 (1975).

    Article  CAS  Google Scholar 

  14. Gesteland, R. F. et al. Cell 7, 381–390 (1976).

    Article  CAS  Google Scholar 

  15. Heindell, H. C., Liu, A., Paddock, G. V., Studnicka, G. M. & Salser, W. A. Cell 15, 43–54 (1978).

    Article  CAS  Google Scholar 

  16. Efstradiatis, A., Kafatos, F. C. & Maniatis, T. Cell 10, 571–585 (1977).

    Article  Google Scholar 

  17. Kohli, J., Kwong, T., Altruda, F., Soll, D. & Wahl, G. J. biol. Chem. 254, 1546–1551 (1979).

    CAS  PubMed  Google Scholar 

  18. Grebanier, A. E., Coen, D. M., Rich, A. & Bogorad, L. J. Cell Biol. 78, 734–746 (1978).

    Article  CAS  Google Scholar 

  19. O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Panyim, S. & Chalkley, R. Archs Biochem. Biophys. 130, 337–346 (1969).

    Article  CAS  Google Scholar 

  21. Lodish, H. F. J. biol. Chem. 246, 7131–7138 (1971).

    CAS  PubMed  Google Scholar 

  22. Roy, K. L., Bloom, A. & Soll, D. in Procedures Nucleic Acid Res. 2, 524–541 (1971).

    Google Scholar 

  23. Joseph, D. R. & Muench, K. H. J. biol. Chem. 246, 7602–7609 (1971).

    CAS  PubMed  Google Scholar 

  24. Buckingham, R. H. Nucleic Acids Res. 3, 965–975 (1976).

    Article  CAS  Google Scholar 

  25. Hirashima, A., Harigai, H. & Watanabe, I. Biochem. biophys. Res. Commun. 88, 1046–1051 (1979).

    Article  CAS  Google Scholar 

  26. Ravetch, J. V. et al. Proc. natn. Acad. Sci. U.S.A. 75, 2195–2198 (1979).

    Article  ADS  Google Scholar 

  27. Philipson, L. et al. Cell 13, 189–199 (1978).

    Article  CAS  Google Scholar 

  28. Murphy, E. C. Jr., Kopchick, J. J., Watson, K. F. & Arlinghaus, R. B. Cell 13, 359–369 (1978).

    Article  CAS  Google Scholar 

  29. Weiss, S. R. Cell 15, 607–614 (1978).

    Article  CAS  Google Scholar 

  30. Weatherall, D. J. & Clegg, J. B. Cell 16, 467–479 (1979).

    Article  CAS  Google Scholar 

  31. Lu, P. & Rich, A. J. molec. Biol. 58, 513–531 (1971).

    Article  CAS  Google Scholar 

  32. Crick, F. H. C. Science 204, 264–271 (1979).

    Article  ADS  CAS  Google Scholar 

  33. Miller, J. H., Ganem, D., Lu, P. & Schmitz, A. J. molec. Biol. 109, 275–301 (1977).

    Article  CAS  Google Scholar 

  34. Soll, L. J. molec. Biol. 86, 233–243 (1974).

    Article  CAS  Google Scholar 

  35. Smith, J. D., Abelson, J. N., Clark, B. F. C., Goodman, H. M. & Brenner, S. Cold Spring Harb. Symp. quant. Biol. 31, 479–485 (1966).

    Article  CAS  Google Scholar 

  36. Nirenberg, M. W. in Meth. Enzym. 6, 17–23 (1963).

    Google Scholar 

  37. Abelson, J. N. et al. J. molec. Biol. 47, 15–28 (1970).

    Article  CAS  Google Scholar 

  38. Walker, R. T. & RajBhandary, U. L. J. biol. Chem. 247, 4879–4892 (1972).

    CAS  PubMed  Google Scholar 

  39. Calander, B. & Berg, P. Procedures Nucleic Acids Res. 1, 384–399 (1966).

    Google Scholar 

  40. Pelham, H. R. B. & Jackson, R. J. EUR. J. Biochem. 67, 247–256 (1976).

    Article  CAS  Google Scholar 

  41. Villa-Kamaroff, L., McDowell, M., Baltimore, D. & Lodish, H. F. Meth. Enzym. 30, 709–723 (1974).

    Article  Google Scholar 

  42. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  43. Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    Article  CAS  Google Scholar 

  44. Weber, K. & Osborn, M. J. biol Chem. 244, 4406–4412 (1969).

    CAS  PubMed  Google Scholar 

  45. Bonner, W. M. & Stedman, J. D. Analyt. Biochem. 89, 247–256 (1978).

    Article  CAS  Google Scholar 

  46. Chlumecka, V., von Tigerstrom, M., D'Obrenan, P. & Smith, C. J. J. biol Chem. 244, 5481–5488 (1969).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139

    Alfred I. Geller & Alexander Rich

Authors
  1. Alfred I. Geller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexander Rich
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Geller, A., Rich, A. A UGA termination suppression tRNATrp active in rabbit reticulocytes. Nature 283, 41–46 (1980). https://doi.org/10.1038/283041a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/283041a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing