Abstract
Ankyrin, the membrane attachment protein for human erythrocyte spectrin, is tightly linked in a 1:1 molar ratio with band 3 in detergent extracts of spectrin-depleted membranes. Ankyrin-linked band 3, which represents 10–15% of the total band 3, spans the membrane, and is nearly identical to the major band 3 by peptide analysis. Spectrin binds to solubilised ankyrin-linked band 3, but not to free band 3. A portion of band 3 remains firmly associated with detergent-extracted cytoskeletal proteins. It is concluded that a fraction of band 3 is attached to the erythrocyte cytoskeleton through association with ankyrin, which in turn is bound to spectrin.
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Bennett, V., Stenbuck, P. The membrane attachment protein for spectrin is associated with band 3 in human erythrocyte membranes. Nature 280, 468–473 (1979). https://doi.org/10.1038/280468a0
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DOI: https://doi.org/10.1038/280468a0
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