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Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance

Nature volume 279, pages 778–781 (1979)Cite this article

Abstract

A cloned BamH1-generated fragment of ColV,I-K94 increased the virulence of Escherichia coli, causing an approximately 100-fold reduction in LD50 for chicks. A genetic determinant f or resistance to the bactericidal effects of serum was mapped to a 5,300 base-pair sequence within the fragment. Neither colicin V nor immunity to colicin V affected the pathogenicity of E. coli for chicks.

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References

  1. Smith, H. W. in Acute Diarrhoea in Childhood (eds Elliot, K. & Knight, J.) 44–72 (Elsevier, Amsterdam, 1976).

    Google Scholar 

  2. Smith, H. W. & Halls, S. J. gen. Microbiol. 52, 319–334 (1968).

    Article  Google Scholar 

  3. Ørskov, I. & Ørskov, F. J. Bact. 91, 69–75 (1966).

    PubMed  Google Scholar 

  4. Smith, H. W. J. gen. Microbiol. 83, 95–111 (1974).

    Article  CAS  Google Scholar 

  5. Smith, H. W. & Huggins, M. B. J. gen. Microbiol. 92, 335–350 (1976).

    Article  CAS  Google Scholar 

  6. Braude, A. I. & Siemienski, J. S. J. clin. Invest. 47, 1763–1773 (1965).

    Article  Google Scholar 

  7. Smith, H. W. J. med. Microbiol. 10, 479–482 (1977).

    Article  CAS  Google Scholar 

  8. Hardy, K. G. Bact. Rev. 39, 464–515 (1975).

    CAS  PubMed  Google Scholar 

  9. Bolivar, F. et al. Gene 2, 95–113 (1977).

    Article  CAS  Google Scholar 

  10. Kahn, P. L. & Helinski, D. R. J. Bact. 88, 1573–1579 (1964).

    CAS  PubMed  Google Scholar 

  11. Nagel de Zwaig, R. & Antón, D. N. Biochem. biophys. Res. Commun. 17, 358–362 (1964).

    Article  CAS  Google Scholar 

  12. MacFarren, A. C. & Clowes, R. C. J. Bact. 84, 365–377 (1977).

    Google Scholar 

  13. Fredericq, P. & Joiris, E. C. r. Séanc. Soc. Biol. 144, 435–437 (1950).

    CAS  Google Scholar 

  14. Davidson, N. et al. in Microbiology—1974 (ed. Schlessinger, D.) 55–65 (American Society for Microbiology, Washington, 1975).

    Google Scholar 

  15. Guyer, M. S. J. molec. Biol. 126, 347–365 (1978).

    Article  CAS  Google Scholar 

  16. Cohen, S. N., Chang, A. C. Y. & Hsu, L. Proc. natn. Acad. Sci. U.S.A. 69, 2110–2114 (1972).

    Article  ADS  CAS  Google Scholar 

  17. Berg, D. E., Davies, J., Allet, B. & Rochaix, J.-D. Proc. natn. Acad. Sci. U.S.A. 72, 3628–3632 (1975).

    Article  ADS  CAS  Google Scholar 

  18. Tessman, I. Virology 35, 330–333 (1968).

    Article  CAS  Google Scholar 

  19. Hirota, Y. Proc. natn. Acad. Sci. U.S.A. 46, 57–64 (1960).

    Article  ADS  CAS  Google Scholar 

  20. Bazaral, M. & Helinski, D. R. J. molec. Biol. 36, 185–194 (1968).

    Article  CAS  Google Scholar 

  21. Jacob, F., Brenner, S. & Cuzin, F. Cold Spring Harb. Symp. quant. Biol. 28, 329–348 (1963).

    Article  CAS  Google Scholar 

  22. Davis, B. D. & Mingioli, E. S. J. Bact. 60, 17–28 (1950).

    CAS  PubMed  Google Scholar 

  23. Meynell, E., Meynell, G. G. & Datta, N. Bact. Rev. 32, 55–83 (1968).

    CAS  PubMed  Google Scholar 

  24. Hardy, K. G., Meynell, G. G., Dowman, J. E. & Spratt, B. G. Molec. gen. Genet. 125, 217–230 (1973).

    Article  CAS  Google Scholar 

  25. Warren, G. J., Twigg, A. J. & Sherratt, D. J. Nature 274, 259–261 (1978).

    Article  ADS  CAS  Google Scholar 

  26. Gratia, A. C. r. Séanc. Soc. Biol. 93, 1040–1041 (1925).

    Google Scholar 

  27. Smith, H. W. & Parsell, Z. J. gen. Microbiol. 87, 129–140 (1975).

    Article  CAS  Google Scholar 

  28. Mooi, F. R., de Graaf, F. K. & van Embden, J. D. A. Nucleic Acids Res. 6, 849–865 (1979).

    Article  CAS  Google Scholar 

  29. Horák, V. & Lanc, A. Folia Microbiol. 18, 332–340 (1973).

    Article  Google Scholar 

  30. Minshew, B. et al. J. infect. Dis. 137, 648–654 (1978).

    Article  CAS  Google Scholar 

  31. Taylor, P. W. & Hughes, C. Infect. Immun. 22, 10–17 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  32. Sharp, P. A., Cohen, S. N. & Davidson, N. J. molec. Biol. 75, 235–255 (1973).

    Article  CAS  Google Scholar 

  33. Kennedy, N. et al. Nature 270, 580–585 (1977).

    Article  ADS  CAS  Google Scholar 

  34. Willetts, N. S. & McIntire, S. J. molec. Biol. 126, 525–549 (1978).

    Article  CAS  Google Scholar 

  35. Greene, P. J. et al. in Methods in Molecular Biology Vol. 7 (ed. Wickner, R. B.) 87–111 (Dekker, New York, 1974).

    Google Scholar 

  36. Phillipsen, P., Kramer, R. A. & Davis, R. W. J. molec. Biol. 123, 371–386 (1978).

    Article  Google Scholar 

  37. Davies, J. K. & Reeves, P. J. Bact. 123, 96–101 (1975).

    CAS  PubMed  Google Scholar 

  38. Finney, D. J. Probit Analysis 3rd edn (Cambridge University Press, 1971).

    MATH  Google Scholar 

Download references

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Authors and Affiliations

  1. Biological Laboratory, University of Kent at Canterbury, Canterbury, UK

    M. M. Binns, D. L. Davies & K. G. Hardy

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  1. M. M. Binns
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  2. D. L. Davies
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  3. K. G. Hardy
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Binns, M., Davies, D. & Hardy, K. Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance. Nature 279, 778–781 (1979). https://doi.org/10.1038/279778a0

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