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Protein disk of tobacco mosaic virus at 2.8 Å resolution showing the interactions within and between subunits

Nature volume 276, pages 362–368 (1978)Cite this article

Abstract

Protein subunits in the two layers of the disk of tobacco mosaic virus have very similar conformations. Much of the bonding between subunits is polar, including salt-bridge systems. Arginine residues play a prominent part here and elsewhere. Interactions within each layer involve groups whose contacts can be adjusted to allow the transition from disk to virus helix. Aromatic clusters within each molecule are linked in a hydrophobic girdle encircling each ring.

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References

  1. Caspar, D. L. D. Adv. Protein Chem. 18, 37–118 (1963).

    Article  CAS  Google Scholar 

  2. Butler, P. J. G. & Klug, A. Nature new Biol. 229, 47–50 (1971).

    Article  CAS  Google Scholar 

  3. Finch, J. T., Leberman, R., Chang, Y.-S. & Klug, A. Nature 212, 349–350 (1966).

    Article  ADS  CAS  Google Scholar 

  4. Finch, J. T. & Klug, A. Phil. Trans. R. Soc. B261, 211–219 (1971).

    Article  CAS  Google Scholar 

  5. Champness, J. N., Bloomer, A. C., Bricogne, G., Butler, P. J. G. & Klug, A. Nature 259, 20–24 (1976).

    Article  ADS  CAS  Google Scholar 

  6. Butler, P. J. G. et al. Proc. 3rd John Innes Symp., 101–110 (1976).

  7. Zimmern, D. & Butler, P. J. G. Cell 11, 455–462 (1977).

    Article  CAS  Google Scholar 

  8. Zimmern, D. Cell 11, 463–482 (1977).

    Article  CAS  Google Scholar 

  9. Stubbs, G. J., Warren, S. G. & Holmes, K. C. Nature 267, 216–221 (1976).

    Article  ADS  Google Scholar 

  10. Leberman, R., Finch, J. T., Gilbert, P. F. C., Witz, J. & Klug, A. J. molec. Biol. 86, 179–182 (1974).

    Article  CAS  Google Scholar 

  11. Finch, J. T., Gilbert, P. F. C., Klug, A. & Leberman, R. J. molec. Biol. 86, 183–192 (1974).

    Article  CAS  Google Scholar 

  12. Gilbert, P. F. C. & Klug, A. J. molec. Biol. 86, 193–207 (1974).

    Article  CAS  Google Scholar 

  13. Arndt, U. W. & Wonacott, A. J. (eds) The Rotation Method in Crystallography (North-Holland, Amsterdam, 1977).

  14. Mallett, J. F. W., Champness, J. N., Faruqi, A. R. & Gossling, T. H. J. Phys. E: Sci. Instrum. 10, 351–358 (1977).

    Article  ADS  CAS  Google Scholar 

  15. Jack, A. Acta crystallogr. A29, 545–554 (1973).

    Article  CAS  Google Scholar 

  16. Bricogne, G. Acta crystallogr. A30, 395–405 (1974).

    Article  CAS  Google Scholar 

  17. Bricogne, G. Acta crystallogr. A32, 832–847 (1976).

    Article  Google Scholar 

  18. Richards, F. M. J. molec. Biol. 37, 225–230 (1968).

    Article  CAS  Google Scholar 

  19. Diamond, R. Proc. IUPAB Symp. biomolec. Struct., Madras (1978).

  20. Dayhoff, M. O., Atlas of Protein Sequence and Structure, Vol.5, D283(National Biomedical Research Foundation, Washington, 1972).

    Google Scholar 

  21. Takano, T. J. molec. Biol. 110, 537–568 (1977).

    Article  CAS  Google Scholar 

  22. Jardetzky, O., Akasaka, K., Vogel, D., Morris, S. & Holmes, K. C. Nature 273, 564–566 (1978).

    Article  ADS  CAS  Google Scholar 

  23. Durham, A. C. H., Finch, J. T. & Klug, A. Nature new Biol. 229, 37–42 (1971).

    Article  CAS  Google Scholar 

  24. Graham, J. & Butler, P. J. G. Eur. J. Biochem. 83, 523–528 (1978).

    Article  CAS  Google Scholar 

  25. Rees, M. W. & Short, M. N. Biochim. biophys. Acta 393, 15–23 (1975).

    Article  CAS  Google Scholar 

  26. Graham, J. thesis, Univ. Cambridge (1977).

  27. Wilson, T. M. A., Perham, R. N., Finch, J. T. & Butler, P. J. G. FEBS Lett. 64, 285–289 (1976).

    Article  CAS  Google Scholar 

  28. Graham, J. & Butler, P. J. G. Eur. J. Biochem. (in the press).

  29. Franklin, R. E. & Holmes, K. C. Acta crystallogr. 11, 213–220 (1958).

    Article  CAS  Google Scholar 

  30. Huber, R. & Bode, W. Acc. Chem. Res. 11, 114–122 (1978).

    Article  CAS  Google Scholar 

  31. Durham, A. C. H. FEBS Lett. 25, 147–152 (1972).

    Article  CAS  Google Scholar 

  32. Lauffer, M. A., Entropy Driven Processes in Biology (Springer, Heidelberg, 1975).

    Book  Google Scholar 

  33. Butler, P. J. G., Finch, J. T. & Zimmern, D. Nature 265, 217–219 (1977).

    Article  ADS  CAS  Google Scholar 

  34. Lebeurier, G., Nicolaieff, A. & Richards, K. E. Proc. natn. Acad. Sci. U.S.A. 74, 149–153 (1977).

    Article  ADS  CAS  Google Scholar 

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Author notes

  1. J. N. Champness

    Present address: Wellcome Research Laboratories, Langley Court, Beckenham, Kent, UK

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK

    A. C. Bloomer, J. N. Champness, G. Bricogne, R. Staden & A. Klug

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  1. A. C. Bloomer
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  2. J. N. Champness
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  3. G. Bricogne
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  4. R. Staden
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  5. A. Klug
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Bloomer, A., Champness, J., Bricogne, G. et al. Protein disk of tobacco mosaic virus at 2.8 Å resolution showing the interactions within and between subunits. Nature 276, 362–368 (1978). https://doi.org/10.1038/276362a0

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