Abstract
WE have developed a long-term bone marrow culture system in which proliferation and differentiation of apparently functionally normal haemopoietic stem cells (CFU-S) can be sustained in vitro for several months1–8. We now find that genetic resistance9,10 or host-versus-graft (H-v-G) reaction, the (possibly) related natural killer (NK) cell activity11 and the ability of cultured cells to reconstitute irradiated allogeneic (non-compatible) recipients (with the subsequent development of G-v-H disease12) are all absent from such cultured cells.
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DEXTER, T., SPOONCER, E. Loss of immunoreactivity in long-term bone marrow culture. Nature 275, 135–136 (1978). https://doi.org/10.1038/275135a0
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DOI: https://doi.org/10.1038/275135a0
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