Abstract
Chimaeric plasmids have been constructed containing a yeast plasmid and fragments of yeast nuclear DNA linked to pMB9, a derivative of the ColEl plasmid from E. coli. Two plasmids were isolated which complement leuB mutations in E. coli. These plasmids have been used to develop a method for transforming a leu2 strain of S. cerevisiae to Leu+ with high frequency. The yeast transformants contained multiple plasmid copies which were recovered by transformation in E. coli. The yeast plasmid sequence recombined intramolecularly during propagation in yeast.
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References
Hinnen, A., Hicks, J. B., Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).
Guerineau, M., Grandchamp, C., Paoletti, C. & Slonimski, P. P. Biochem. biophys. Res. Commun. 42, 550–557 (1971).
Stevens, B. J. & Moustacchi, E. Expl Cell Res. 64, 259–266 (1971).
Bak, A. L., Christiansen, C. & Christiansen, G. Biochim. biophys. Acta 269, 527–530 (1972).
Clark-Walker, G. D. Eur. J. Biochem. 32, 263–267 (1973).
Clark-Walker, G. D. Proc. natn. Acad. Sci. U.S.A. 69, 388–392 (1972).
Beggs, J. D., Guerineau, M. & Atkins, J. F. Molec. gen. Genet. 148, 287–294 (1976).
Petes, T. D. & Williamson, D. H. Cell 4, 249–253 (1975).
Livingston, D. M. & Kupfer, D. M. J. molec. Biol. 116, 249–260 (1977).
Clark-Walker, G. D. & Miklos, G. L. G. Eur. J. Biochem. 41, 359–365 (1974).
Hollenberg, C. P., Degelmann, A., Kustermann-Kuhn, B. & Royer, H. D. Proc. natn. Acad. Sci. U.S.A. 73, 2072–2076 (1976).
Guerineau, M., Grandchamp, C. & Slonimski, P. P. Proc. natn. Acad. Sci. U.S.A. 73, 3030–3034 (1976).
Livingston, D. M. & Klein, H. L. J. Bact. 129, 472–481 (1977).
Livingston, D. M. Genetics 86, 73–84 (1977).
Cameron, J. R., Philippsen, P. & Davis, R. W. Nucleic Acids Res. 4, 1429–1448 (1977).
Bolivar, F., Rodriguez, R. L., Betlach, M. C. & Boyer, H. W. Gene 2, 75–93 (1977).
Guerineau, M., Slonimski, P. P. & Avner, P. R. Biochem. biophys. Res. Commun. 61, 462–469 (1974).
Jackson, D., Symons, R. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 69, 2904–2909 (1972).
Somers, J. M., Amzallag, A. & Middleton, R. B. J. Bact. 113, 1268–1272 (1973).
Borck, K., Beggs, J. D., Brammer, W. J., Hopkins, A. S. & Murray, N. E. Molec. gen. Genet. 146, 199–207 (1976).
Clewell, D. J. Bact. 110, 667–676 (1972).
Clewell, D. D. & Helinski, D. R. Proc. natn. Acad. Sci. U.S.A. 62, 1159–1166 (1969).
Murray, N. E., Brammar, W. J. & Murray, K. Molec. gen. Genet. 150, 53–61 (1977).
Lederberg, E. M. & Cohen, S. N. J. Bact. 119, 1072–1074 (1974).
Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).
Maniatis, T., Jeffrey, A. & Kleid, D. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).
Barnes, W. M. Science 195, 393–394 (1977).
Davidson, E. H., Hough, B. R., Amenson, C. S. & Britten, R. J. J. molec. Biol. 77, 1–23 (1973).
Lobban, P. E. & Kaiser, A. D. J. molec. Biol. 78, 453–471 (1973).
Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 863–877 (1976).
Spizizen, J. Proc. natn. Acad. Sci. U.S.A. 44, 1072–1078 (1958).
Burgess, R. R. J. biol. Chem. 244, 6160–6167 (1969).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
van Solingen, P. & van der Plaat, J. B. J. Bact. 130, 946–947 (1977).
Cryer, D. R., Eccleshall, R. & Marmur, J. Methods in Cell Biology XII, 39–44 (1975).
Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–316 (1977).
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Beggs, J. Transformation of yeast by a replicating hybrid plasmid. Nature 275, 104–109 (1978). https://doi.org/10.1038/275104a0
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DOI: https://doi.org/10.1038/275104a0
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