Abstract
WHILE investigating surface immunoglobulin (Ig) on frog (Rana catesbeiana) lymphocytes by immunofluorescence, we observed, unexpectedly, that several rabbit antisera to frog Ig stained frog thymocytes and all blood cells, including lymphocytes, granulocytes and erythrocytes1. Absorption with frog erythrocytes abolished the staining of thymocytes and granulocytes, indicating that similar antigenic determinants are present on these cell types. Some peripheral lymphocytes were still stained by the absorbed antiserum, but others were unstained. Evidently, after removal of the cross-reactive antibodies, two lymphocyte populations, possibly homologous to mammalian and avian B and T lymphocytes, can be identified on the basis of surface Ig. Antibodies that react with all blood cells were detected in five of eight antisera to frog high molecular weight (HMW) Ig, but not in six antisera to the anti-genically distinct low molecular weight (LMW) Ig. (Rana catesbeiana have three distinct Ig isotypes: an HMW Ig, which seems similar to mammalian IgM, and two highly cross-reactive LMW Igs, which have an uncertain relationship to mammalian Ig classes2,3.) The presence of these cross-reactive antibodies in antisera to HMW Ig may lead to the mistaken identification of Ig on cell surfaces. Absorption of such an antiserum with purified frog HMW Ig and with a non-Ig component of frog plasma, but not with frog LMW Ig, also abolished the staining of thymocytes and granulocytes. The presence of similar determinants on the surfaces of several cell types and on at least two plasma components raised the possibility that a carbohydrate structure might be involved. Experiments supporting this possibility are reported here.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Mattes, M. J. & Steiner, L. A. J. Immun. (in the press).
Green, C. & Steiner, L. A. J. Immun. 117, 364–374 (1976).
Marchalonis, J. J. Immunity in Evolution (Harvard University Press, Cambridge, 1977).
Clamp, J. R. & Hough, L. Biochem. J. 94, 17–24 (1965).
Andersen, B. R., Abele, D. C. & Vannier, W. D. J. Immun. 97, 913–924 (1966).
Lee, Y. C. & Montgomery, R. Archs Biochem. Biophys. 95, 263–270 (1961).
Merler, E., Gatien, J. & DeWilde, G. Nature 251, 652–654 (1974).
Ivanyi, J., Strudwick, L. & Makings, C. Eur. J. Immun. 7, 204–209 (1977).
Herberman, R. B. J. Immun. 104, 805–809 (1970).
Quindlen, E. A., Wood, W. C. & Kornblith, P. L. J. Immun. 118, 1836–1842 (1977).
DuPasquier, L., Weiss, N. & Loor, F. Eur. J. Immun. 2, 366–370 (1972).
Charlemagne, J. & Tournefier, A. in Immunologic Phylogeny (eds Hildemann, W. H. & Benedict, A. A.) 251–255 (Plenum, New York, 1975).
Emmrich, F., Richter, R. F. & Ambrosius, H. Eur. J. Immun. 5, 76–78 (1975).
Warr, G. W., DeLuca, D. & Marchalonis, J. J. Proc. natn. Acad. Sci. U.S.A. 73, 2476–2480 (1976).
Ellis, A. E. & Parkhouse, R. M. E. Eur. J. Immun. 5, 726–728 (1975).
Clem, L. W., McLean, W. E., Shankey, V. T. & Cuchens, M. A. Devl comp. Immun. 1, 105–118 (1977).
Hammerling, U., Mack, C. & Pickel, H. G. Immunochemistry 13, 525–531 (1976).
Warner, N. L. Adv. Immun. 19, 67–216 (1974).
Marchalonis, J. J. Science 190, 20–29 (1975).
Tanaka, N. & Leduc, E. H. J. Immun. 77, 198–212 (1956).
Siddiqui, B. & Hakomori, S. J. biol. Chem. 246, 5766–5769 (1971).
Watkins, W. M. Science 152, 172–181 (1966).
Hakomori, S. & Kobata, A. in The Antigens Vol. 2 (ed. Sela, M.) 79–140 (Academic, New York, 1974).
Putnam, F. W., Florent, G., Paul, C., Shinoda, T. & Shimizu, A. Science 182, 287–291 (1973).
Watanabe, S., Barnikol, H. U., Horn, J., Bertram, J. & Hilschmann, N. Hoppe-Seyler's Z. physiol. Chem. 354, 1505–1509 (1973).
Shimizu, A., Putnam, F. W., Paul, C., Clamp, J. R. & Johnson, I. Nature new Biol. 231, 73–76 (1971).
Hickman, S., Kornfeld, R., Osterland, C. K. & Kornfeld, S. J. biol. Chem. 247, 2156–2163 (1972).
Tarentino, A. L., Plummer, T. H., Jr & Maley, F. Biochemistry 14, 5516–5523 (1975).
Hogg, N. W. & Greaves, M. F. Immunology 22, 967–980 (1972).
Mihaesco, C. & Seligmann, M. Immunochemistry 5, 457–469 (1968).
Yemm, E. W. & Cocking, E. C. Analyst, Lond. 80, 209–213 (1955).
Yurewicz, E. C., Ghalambor, M. A. & Heath, E. C. J. biol. Chem. 246, 5596–5606 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MATTES, M., STEINER, L. Antisera to frog immunoglobulins cross-react with a periodate-sensitive cell surface determinant. Nature 273, 761–763 (1978). https://doi.org/10.1038/273761a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/273761a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.