Abstract
A set of partially overlapping DNA restriction fragments that support promoter-dependent transcription of the tRNA1Tyr gene of Escherichia coli has been used to study site-specific termination in vitro. Transcription termination occurs at a specific site 224–226 nucleotides beyond the end of the structural gene and is completely dependent on ρ-factor. Certain features of this site suggest differences from other termination sites previously studied. A role for specific sequence recognition is suggested.
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References
RNA Polymerase (eds Losick, R. & Chamberlin, M.) (1976).
Burgess, R. R., Travers, A. A., Dunn, J. J. & Bautz, E. K F. Nature 221, 43 (1969).
Roberts, J. W. Nature 224, 1168–1174 (1969).
Das, A., Court, D. & Adhya, S. Proc. natn. Acad. Sci. U.S.A. 73, 1959–1963 (1976).
Lebowitz, P., Weissman, S. H. & Radding, C. M. J. biol. Chem. 246, 5120–5139 (1971).
Blattner, F. R. & Dahlberg, J. E. Nature new Biol. 237, 227–232 (1972).
Bertrand, K. et al. Science 189, 22–26 (1975).
Pieczenik, G., Barrell, B. G. & Gefter, M. L. Archs Biochem. Biophys. 152, 152–165 (1972).
Millette, R. L., Trotter, C. D., Herrlich, P. & Schweiger, M. Cold Spring Harb. Symp. quant. Biol. 35, 135–142 (1970).
Landy, A., Foeller, C. & Ross, W. Nature 249, 738–742 (1974).
Küpper, H., Contreras, R., Landy, A. & Khorana, H. G. Proc. natn. Acad. Sci. U.S.A. 72, 4754–4758 (1975).
Maizels, N. Proc. natn. Acad. Sci. U.S.A. 70, 3585–3589 (1973).
Beckmann, J. S. & Daniel, V. Biochemistry 13, 4058–4062 (1974).
Fournier, M. J., Webb, E. & Tang, S. Biochemistry 16, 3608–3618 (1977).
Goldberg, A. R. & Hurwitz, J. J. biol. Chem. 247, 5637–5645 (1972).
Rosenberg, M., Weissman, S. & deCrombrugghe, B. J. biol. Chem. 250, 4755–4764 (1975).
Howard, B. H., deCrombrugghe, B. & Rosenberg, M. Nucleic Acids Res. 4, 827–842 (1977).
Roberts, J. W. RNA Polymerase (eds Losick, R. & Chamberlin, M.), 247–271 (1976).
Egan, J. & Landy, A. J. biol. Chem. (in the press).
Rosenberg, M. Nucleic Acids Res. 1, 653–671 (1974).
Rosenberg, M., Court, D., Wulff, D., Shimatake, H. & Brady, C. Nature 272, 414–423 (1978).
Lee, F. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 74, 4365–4369 (1977).
Sogin, M., Pace, N., Rosenberg, M. & Weissman, S. J. biol. Chem. 251, 3480–3488 (1976).
Court, D., Wulff, D., Brady, C. & Rosenberg, M. J. molec. Biol. (submitted).
Rosenberg, M., deCrombrugghe, B. & Musso, R. Proc. natn. Acad. Sci. U.S.A. 73, 717–721 (1976).
Gilbert, W RNA Polymerase (eds Losick, R. & Chamberlin, M.) 193–205 (1976).
Korn, L. J., Queen, C. L. & Wegman, M. N. Proc. natn. Acad. Sci. U.S.A. 74, 4401–4405 (1977).
Minkley, E. G. & Pribnow, D. J. molec. Biol. 77, 255–277 (1973).
Pribnow, D. Proc. natn. Acad. Sci. U.S.A. 72, 784–788 (1975).
Ikeda, H. Nature new Biol. 234, 198–201 (1971).
Bikoff, E. K., LaRue, B. F. & Gefter, M. L. J. biol. Chem. 250, 6248–6255 (1975).
Russell, R. L. et al. J. molec. Biol. 47, 1–13 (1970).
Altman, S. & Smith, J. D. Nature new Biol. 233, 35–39 (1971).
Burgess, R. R. & Jendrisak, J. J. Biochemistry 14, 4634–4638 (1975).
Sanger, F., Brownlee, G. G. & Barrell, B. G. J. molec. Biol. 13, 373–398 (1965).
Brownlee, G. G. & Sanger, F. Eur. J. Biochem. 11, 395–399 (1969).
Brownlee, G. G. in Laboratory Techniques in Biochemistry: Determination of Sequences in RNA (eds Work, T. S. & Work, E.) (1972).
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Küpper, H., Sekiya, T., Rosenberg, M. et al. A ρ-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli. Nature 272, 423–428 (1978). https://doi.org/10.1038/272423a0
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DOI: https://doi.org/10.1038/272423a0
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