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Functional stabilisation of HeLa cell histone messenger RNAs injected into Xenopus oocytes by 3′- OH polyadenylation

Nature volume 271, pages 572–573 (1978)Cite this article

Abstract

WE have shown that, when injected into Xenopus oocytes, poly (A) free globin messenger RNA is translated for a short period of time and then rapidly degraded1–3, while, under the same conditions, native mRNA shows a marked stability1,2,4. The poly (A) segment itself is responsible for the stability of native globin mRNA, since its readdition to previously de-adenylated mRNA restores the stability of the message5. We have shown also that the poly (A) stretch must contain a minimum number of adenylate residues to ensure its protective function6. It was interesting to see whether the concept of the stabilisation of eukaryotic mRNAs by 3′-OH polyadenylation is general. This can be done by looking at the effect of the presence of a 3′-OH poly (A) segment on the stability of various mRNAs. Here we study the stability of HeLa cell poly (A)-free histone mRNAs injected into Xenopus oocytes, and the effect of 3′-OH polyadenylation on this stability.

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Authors and Affiliations

  1. Department of Molecular Biology, University of Brussels, 1640, Rhode St-Genèse, Belgium

    G. HUEZ, G. MARBAIX, E. HUBERT & Y. CLEUTER

  2. Institute of Physiological Chemistry I, Philipps University, Lahnberge, 355, Marburg/Lahn, West Germany

    D. GALLWITZ

  3. Mergenthaler Laboratory of Biology, The Johns Hopkins University, Baltimore, Maryland

    E. WEINBERG

  4. Laboratories of Molecular Biology and of Physiological Chemistry, University of Ghent, 9000, Ghent, Belgium

    R. DEVOS

Authors
  1. G. HUEZ
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  2. G. MARBAIX
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  3. E. HUBERT
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  4. Y. CLEUTER
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  5. D. GALLWITZ
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  6. E. WEINBERG
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  7. R. DEVOS
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HUEZ, G., MARBAIX, G., HUBERT, E. et al. Functional stabilisation of HeLa cell histone messenger RNAs injected into Xenopus oocytes by 3′- OH polyadenylation. Nature 271, 572–573 (1978). https://doi.org/10.1038/271572a0

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