Abstract
XENOIMMUNISATION with human cells or isolated molecules found on the surface of human cells results in the production of antibodies to a variety of human alloantigens (refs 1–3 and unpublished results). The genetic loci which specify the antigens being recognised can then be determined by using rodent–human hybrids containing various human chromosomes as target cells. In this manner, chromosome 11 has been found to include genes coding for a series of antigens designated AL (refs 1, 4) or HSA-1 (ref. 2) and chromosome 15 to code for β2 microglobulin3. Murine–human somatic cell hybrids with one or a limited number of human chromosomes have been used by us and by others to obtain antisera in mice syngeneic to the mouse parental cells reactive with antigens coded for by specific human chromosomes2,5–7. The advantage of this method is that antisera generated are specific for the antigen(s) coded for by a particular human chromosome and the reactivity is to a limited number of human cell surface antigens. This allows for both systematic genetic mapping of these antigens and detection of previously unknown cell surface molecules. Chromosome 6 has been shown both by pedigree analysis8 and by the use of interspecific somatic cell hybrid target cells9 to be the location of the genes coding for the serologically defined antigens (HLA) of the major histocompatibility complex (MHC). We have used a human–mouse somatic cell hybrid containing several human chromosomes, including chromosome 6, to immunise mice syngeneic to the mouse parental cell. The reactivity of the absorbed antiserum has been analysed both serologically and by sodium dodecyl sulphate polyacrylamide gel electrophoresis after immunoprecipitation. Evidence is presented here to show that mice immunised with somatic cell hybrids containing human chromosome 6 produce antibody which reacts with HLA as well as with other human lymphocyte cell surface antigens.
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ADEN, D., MAUSNER, R. & KNOWLES, B. Production of HLA antibody in mice immunised with syngeneic mouse–human hybrid cells containing human chromosome 6. Nature 271, 375–377 (1978). https://doi.org/10.1038/271375a0
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DOI: https://doi.org/10.1038/271375a0
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