Abstract
Every malignant tumour examined contains aberrant tRNA methyltransferases and a few tRN As which are absent from the normal tissue of origin. To determine whether tumour-specific tRN As have different modifications from those in normal tissue, we purified the most frequently occurring tumour-specific isoaccepting tRNA from two malignant tissues. The isoaccepting phenylalanine tRNA from Novikoff hepatoma and Ehrlich ascites cells both contain two supernumerary methylated bases. One of these 1-methylguanine, is absent from the phenylalanine tRNA of normal rat, mouse, rabbit and calf liver. An increase in the levels of 5-methylcytidine and dihydrouridine was also detected.
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Kuchino, Y., Borek, E. Tumour-specific phenylalanine tRNA contains two supernumerary methylated bases. Nature 271, 126–129 (1978). https://doi.org/10.1038/271126a0
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DOI: https://doi.org/10.1038/271126a0
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