Abstract
THE genome of Rous sarcoma virus (RSV) is a 30-40S RNA of 10,000 nucleotides coding for four known genes1 which map in the order 5′-gag, pol, env, src, poly(A)-3′ (refs 2, 3). This 30-40S RNA serves as mRNA for a gag precursor protein and perhaps a gag-pol precursor-polyprotein when translated in vitro4,5. The env gene, however, may be translated from a smaller intracellular messenger. Poly(A)-containing 20-24S cellular RNA from infected cells can, upon microinjection, complement an env-defective virus6 and may also direct in vitro synthesis of a protein that is serologically related to the env gene product7. Moreover, hybridisation with DNA complementary (cDNA) to env and src sequences of virion RNA detects discrete classes of subgenomic poly(A)-containing RNAs present in infected cells as steady state species, while gag and pol-specific cDNAs hybridise only to full sized RNA8,9.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Baltimore, D. Cold Spring Harbor Symp. quant. Biol. 39, 1187–1200 (1975).
Wang, L. H., Duesberg, P., Mellon, P. & Vogt, P. K. Proc. natn. Acad. Sci. U.S.A. 73, 1073–1077 (1976).
Wang, L. H., Galehouse, D., Mellon, P., Duesberg, P., Mason, W. S. & Vogt, P. K. Proc. natn. Acad. Sci. U.S.A. 73, 3952–3956 (1976).
von der Helm, K. & Duesberg, P. H. Proc. natn. Acad. Sci. U.S.A. 72, 614–618 (1975).
Kerr, I. M., Olshevsky, U., Lodish, H. G. & Baltimore, D. J. Virol 18, 627–635 (1976).
Stacey, D. W., Allfrey, V. G. & Hanafusa, H. Proc. natn. Acad. Sci. U.S.A. 74, 1614–1618 (1977).
Pawson, T., Harvey, R. & Smith, A. E. Nature 268, 416–420 (1977).
Hayward, W. S. J. Virol. 24, 47–63 (1977).
Bishop, J. M., Deng, C-T., Mahy, B. W. J., Quintrell, N., Stavnezer, E. & Varmus, H. in Animal Virology (eds Baltimore, D., Huang, A. S. & Fox, C. F.) 4, 1–20 (Academic Press, New York, 1976).
Chow, L. T., Gelinas, R. E., Broker, T. R. & Roberts, R. J. Cell 12, 1–8 (1977).
Wang, L. H., Duesberg, P., Beemon, K. & Vogt, P. K. J. Virol. 16, 1051–1070 (1975).
Wang, L. H. & Duesberg, P. J. Virol. 14, 1515–1529 (1974).
Beemon, K. L. & Keith, J. M. in Animal Virology (eds Baltimore. D., Huang, A. S. & Fox, C. F.) 4, 97–105 (Academic, New York, 1976).
Wang, L. H., Duesberg, P. H., Robins, T., Yakota, H. & Vogt, P. K. Virology 82, 472–492 (1977).
Friedrich, R., Kung, H.-J., Baker, B., Varmus, H., Goodman, H. & Bishop, J. M. Virology 79, 198–215 (1977).
Coffin, J. & Haseltine, W. Proc. natn. Acad. Sci. U.S.A. 74, 1908–1912 (1977).
Collett, M. S. & Faras, A. J. Proc. natn. Acad. Sci. U.S.A. 73, 925–932 (1976).
Shine, J., Czernilofsky, P., Friedrich, R., Bishop, J. M. & Goodman, H. M. Proc. natn. Acad. Sci. U.S.A. 74, 1473–1477 (1977).
Gilham, P. T. J. Am. Chem. Soc. 86, 4982–4985 (1964).
Coffin, J. M., Parsons, J. T., Rymo, L., Haroz, R. K. & Weissmann, C. J. molec. Biol. 86, 373–396 (1974).
Junghans, R., Duesberg, P. & Knight, C. A. Proc. natn. Acad. Sci. U.S.A. 72, 4895–4899 (1975).
Cashion, L. M., Joho, R. H., Planitz, M. A., Billeter, W. A. & Weissmann, C. Nature 262, 186–190 (1977).
Beemon, K., Duesburg, P. & Vogt, P. K., Proc. natn. Acad. Sci. U.S.A. 71, 4254–4258 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MELLON, P., DUESBERG, P. Subgenomic, cellular Rous sarcoma virus RNAs contain oligonucleotides from the 3′ half and the 5′ terminus of virion RNA. Nature 270, 631–634 (1977). https://doi.org/10.1038/270631a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/270631a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
