Abstract
Concentrations of concanavalin A or the calcium ionophore A23187 that are optimal for the transformation of pig or mouse lymphocytes do not normally cause a measurable increase in calcium influx compared with unstimulated cells. If the cells are treated with the mitogens in conditions where a measurable increase in calcium influx occurs, no stimulation of the cells can occur while the flux is maintained. If any early influx of extracellular calcium is necessary for stimulation, then a much smaller increase in the total concentration of cellular calcium than reported previously is sufficient to allow the entry of lymphocytes into the cell cycle.
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Hesketh, T., Smith, G., Houslay, M. et al. Is an early calcium flux necessary to stimulate lymphocytes?. Nature 267, 490–494 (1977). https://doi.org/10.1038/267490a0
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DOI: https://doi.org/10.1038/267490a0
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