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Colchicine inhibits phosphatidylinositol turnover induced in lymphocytes by concavalin A

Nature volume 265, pages 741–742 (1977)Cite this article

Abstract

RECENTLY colchicine has been shown to affect various membrane properties such as the lateral mobility of receptors and other surface membrane markers and the agglutination of different cell types. Many of the described phenomena also involve concanavalin A (con A); for example, phagocytosis by rabbit polymorphonuclear leukocytes (PMNs) leads to a selective depletion of con A binding sites which is prevented by colchicine1, con A inhibition of lymphocyte immunoglobulin receptor capping is reversed by colchicine2–4 and con A-induced aggregation of PMNs, fibroblasts and hepatoma cells is inhibited by colchicine5–7. In addition to these effects on membrane properties, colchicine also has been shown to inhibit con A-induced lymphocyte transformation at an early stage in the sequence of events leading to thymidine incorporation and blast transformation8,9. These various considerations led us to determine if colchicine could alter phosphatidylinositol (PI) turnover in lymphocytes since it is known that increased PI turnover is an early event induced by con A and other mitogenic lectins10–12.

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References

  1. Oliver, J. M., Ukena, T. E., and Berlin, R. D., Proc. natn. Acad. Sci. U.S.A., 71, 394–398 (1974).

    Article  ADS  CAS  Google Scholar 

  2. Edelman, G. M., Yahara, I., and Wang, J. L., Proc. natn. Acad. Sci. U.S.A., 70, 1442–1446 (1973).

    Article  ADS  CAS  Google Scholar 

  3. Yahara, I., and Edelman, G. M., Nature, 246, 152–155 (1973).

    Article  ADS  CAS  Google Scholar 

  4. Unanue, E. R., and Karnovsky, M. J., J. exp. Med., 140, 1207–1220 (1974).

    Article  CAS  Google Scholar 

  5. Berlin, R. D., and Ukena, T. E., Nature new Biol., 238, 120–122 (1972).

    Article  CAS  Google Scholar 

  6. Yin, H. H., Ukena, T. E., and Berlin, R. D., Science, 178, 867–868 (1972).

    Article  ADS  CAS  Google Scholar 

  7. Nakamura, J., and Terayama, H., Proc. natn. Acad. Sci. U.S.A., 72, 498–502 (1975).

    Article  ADS  CAS  Google Scholar 

  8. Wang, J. L., Gunther, G. R., and Edelman, G. M., J. Cell Biol., 66, 128–144 (1975).

    Article  CAS  Google Scholar 

  9. Gunther, G. R., Wang, J. L., and Edelman, G. M., Expl Cell Res., 98, 15–22 (1976).

    Article  CAS  Google Scholar 

  10. Fisher, D. B., and Mueller, G. C., Biochim. biophys. Acta, 248, 438–448 (1971).

    Google Scholar 

  11. Masuzawa, Y., Osawa, T., Inoue, K., and Nojima, S., Biochim. biophys. Acta, 326, 339–344 (1973).

    Article  CAS  Google Scholar 

  12. Maino, V. C., Hayman, M. J., and Crumpton, M. J., Biochem. J., 146, 247–252 (1975).

    Article  CAS  Google Scholar 

  13. Feit, H., and Barondes, S. H., J. Neurochem., 17, 1355–1364 (1970).

    Article  CAS  Google Scholar 

  14. Gillespie, E., J. Cell Biol., 50, 544–549 (1971).

    Article  CAS  Google Scholar 

  15. Stadler, J., and Franke, W. W., Nature new Biol., 237, 237–238 (1972).

    Article  CAS  Google Scholar 

  16. Bhattacharyya, B., and Wolff, J., Archs Biochem. Biophys., 167, 264–269 (1975).

    Article  CAS  Google Scholar 

  17. Wunderlich, F., Muller, R., and Speth, V., Science, 182, 1136–1138 (1973).

    Article  ADS  CAS  Google Scholar 

  18. Albertini, D. A., and Clark, J. I., Proc. natn. Acad. Sci. U.S.A., 72, 4976–4980 (1975).

    Article  ADS  CAS  Google Scholar 

  19. Becker, J. S., Oliver, J. M., and Berlin, R. D., Nature, 254, 152–154 (1975).

    Article  ADS  CAS  Google Scholar 

  20. Berlin, R. D., in Microtubules and Microtubule Inhibitors (edit. by Borgers, M., and de Brabander, M.), 327–339 (North-Holland, Amsterdam, 1975).

    Google Scholar 

  21. Quinn, P. J., in Microtubules and Microtubule Inhibitors (edit. by Borgers, M., and de Brabander, M.), 79–90 (North-Holland, Amsterdam, 1975).

    Google Scholar 

  22. Quinn, P. J., Biochem. J., 133, 273–281 (1973).

    Article  CAS  Google Scholar 

  23. Hokin, L. E., Int. Rev. Cytol., 23, 187–208 (1968).

    Article  CAS  Google Scholar 

  24. Hokin, L. E., Ann. N Y Acad. Sci., 165, 695–709 (1969).

    Article  ADS  CAS  Google Scholar 

  25. Michell, R. H., Biochim. biophys. Acta, 415, 81–147 (1975).

    Article  CAS  Google Scholar 

  26. Dawson, R. M. C., Freinkel, N., Jungalwala, F. B., and Clarke, N., Biochem. J., 122, 605–607 (1971).

    Article  CAS  Google Scholar 

  27. Michell, R. H., and Lapetina, E. G., Nature new Biol., 240, 258–260 (1972).

    Article  CAS  Google Scholar 

  28. Tsan, M. F., and Berlin, R. D., J. exp. Med., 134, 1016–1035 (1971).

    Article  CAS  Google Scholar 

  29. Karnovsky, M. L., and Wallach, D. F. H., J. biol. Chem., 236, 1895–1901 (1961).

    CAS  PubMed  Google Scholar 

  30. Sastry, P. S., and Hokin, L. E., J. biol. Chem., 241, 3354–3361 (1966).

    CAS  PubMed  Google Scholar 

  31. Graham, R. C., Karnovsky, M. J., Shafer, A. W., Glass, E. A., and Karnovsky, M. L., J. Cell Biol., 32, 629–647 (1967).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. The John Hopkins University School of Medicine, Division of Clinical Immunology at The Good Samaritan Hospital, 5601 Loch Raven Boulevard, Baltimore, Maryland, 21239

    R. ROBERT SCHELLENBERG & ELIZABETH GILLESPIE

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  1. R. ROBERT SCHELLENBERG
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  2. ELIZABETH GILLESPIE
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SCHELLENBERG, R., GILLESPIE, E. Colchicine inhibits phosphatidylinositol turnover induced in lymphocytes by concavalin A. Nature 265, 741–742 (1977). https://doi.org/10.1038/265741a0

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