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Native collagen is not a substrate for the collagen glucosyltransferase of platelets

Abstract

THE adhesion of platelets to collagen in the subendothelial tissue is generally considered to be the primary step in haemostasis following vascular injury. The biochemical basis of this interaction is not fully understood but it has been suggested by Jamieson and coworkers1,2 that the adhesion of platelets to collagen is mediated by the formation of an enzyme–acceptor complex between a glucosyltransferase on the platelet membrane and incomplete carbohydrate residues present in collagen. We report here studies confirming the presence of collagen glucosyltransferase activity associated with platelets but investigations of the influence of collagen substrate conformation on enzyme activity suggest that the above hypothesis is probably untenable.

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References

  1. Jamieson, G. A., Urban, C. L., and Barber, A. J., Nature new Biol., 234, 5–7 (1971).

    Article  CAS  Google Scholar 

  2. Jamieson, G. A., in Platelets: Production, Function, Transfusion and Storage (edit. by Baldini, M. G., and Ebbe, S.), 171–176 (Grune and Stratton, New York, 1974).

    Google Scholar 

  3. Barber, A. J., and Jamieson, G. A., Biochim. biophys. Acta, 252, 533–545 (1971).

    Article  CAS  Google Scholar 

  4. Barber, A. J., and Jamieson, G. A., Biochem. biophys. Acta, 252, 546–552 (1971).

    Article  CAS  Google Scholar 

  5. Bosmann, H. B., Biochem. biophys. Res. Commun., 43, 1118–1126 (1971).

    Article  CAS  Google Scholar 

  6. Bosmann, H. B., and Eylar, E. H. Biochim. biophys. Res. Commun., 30, 89–94 (1968).

    Article  CAS  Google Scholar 

  7. Bosmann, H. B., and Eylar, E. H., Biochem. biophys. Res. Commun., 33, 340–346 (1968).

    Article  CAS  Google Scholar 

  8. Spiro, R. G., and Spiro, M. J., J. biol. Chem., 246, 4899–4918 (1971).

    CAS  PubMed  Google Scholar 

  9. Spiro, M. J., and Spiro, R. G., J. biol. Chem., 246, 4910–4918 (1971).

    CAS  PubMed  Google Scholar 

  10. Myllylä, R., Risteli, L., and Kivirikko, K. I. Eur. J. Biochem., 52, 401–410 (1975).

    Article  Google Scholar 

  11. Harwood, R., Grant, M. E., and Jackson, D. S., Biochem. J., 152 291–302 (1975).

    Article  CAS  Google Scholar 

  12. Lopaciuk, S., and Solum, N. O., Thrombos. Diathes. haemorrh., 21, 409–418 (1969).

    Article  CAS  Google Scholar 

  13. Myllylä, R., Risteli, L., and Kivirikko, K. I., Eur. J. Biochem., 61, 59–67 (1976).

    Article  Google Scholar 

  14. Al-Mondhiry, H., and Spaet, T. H., Proc. Soc. exp. Biol. Med., 135, 878–882 (1970).

    Article  CAS  Google Scholar 

  15. Smith, D. F., and Jamieson, G. A., Fedn proc., 33, 143 (1974).

    Google Scholar 

  16. Myllyllä, R., Risteli, L., and Kivirikko, K. I. Eur. J. Biochem., 58, 517–521 (1975).

    Article  Google Scholar 

  17. Barber, A. J., and Jamieson, G. A., J. biol. Chem., 245, 6357–6365 (1970).

    CAS  Google Scholar 

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MENASHI, S., HARWOOD, R. & GRANT, M. Native collagen is not a substrate for the collagen glucosyltransferase of platelets. Nature 264, 670–672 (1976). https://doi.org/10.1038/264670a0

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