Abstract
THE spleen-colony assay of Till and McCulloch1, which is a clonal assay2, measures the content of haemopoietic cells in blood-forming tissues. Stem cells (colony forming units in spleen, CPUS) form red cells, granulocytes, platelets and themselves (self-renewal) in these colonies3,4. Worton et al.5 reported that stem cells separated by velocity sedimentation differed in their capacity for self-renewal. Schofield and Lajtha6 reported that CPUS from mice treated with iso-propyl methane sulphonate have a low self-renewal capacity. We have studied the functional capacity of myeloid stem cells from normal mice (NBM) and mice treated with hydroxyurea. CPUS from mice treated with five injections of hydroxyurea (5 HUBM, G.S.H. and N. M. Blackett, unpublished) formed 3 times more CPUS and 2.5 times more colony forming units in culture per original colony (CFUC, a committed precursor of granulopoiesis7) than those from NBM. When these grafted CPUS were retransplanted they still formed three times more CFUC and CPUS per secondary colony than those from NBM (Tables 1 and 2).
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References
Till, J. E., and McCulloch, E. A., Rad. Res., 14, 213–222 (1961).
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McCulloch, E. A., and Till, J. E., Rad. Res., 22, 383–397 (1963).
Worton, R. G., McCulloch, E. A., and Till, J. E., J. exp. Med., 130, 91–103 (1969).
Schofield, R., and Lajtha, L. G., Br. J. Haemat., 25, 195–202 (1973).
Bradley, T. R., and Metcalf, D., Aust. J. exp. Biol. Med. Sci., 44, 287–300 (1966).
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Hodgson, G. S., Bradley, T. R., and Telfer, P. A., Cell Tissue Kinet., 5, 283–288 (1972).
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ROSENDAAL, M., HODGSON, G. & BRADLEY, T. Haemopoietic stem cells are organised for use on the basis of their generation-age. Nature 264, 68–69 (1976). https://doi.org/10.1038/264068a0
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DOI: https://doi.org/10.1038/264068a0
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