Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cells

Nature volume 264, pages 26–31 (1976)Cite this article

Abstract

Normal cells in culture have membrane receptors for epidermal growth factor (EGF); EGF stimulates cells to divide by binding to these receptors. Cells transformed by murine and feline sarcoma viruses rapidly lose the ability to bind EGF, whereas cells transformed by the DNA tumour viruses, polyoma and SV40, or infected with non-transforming RNA tumour viruses have normal levels of functional EGF receptors. The results suggest that a produce of the sarcoma virus genome specifically changes cell EGF receptors; the sarcoma gene product may, then, be functionally related to EGF.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Gross, L., in Oncogenic Viruses, second ed. (Pergamon Press, Oxford, 1970).

    Google Scholar 

  2. Tooze, J., in The Molecular Biology of Tumour Viruses (Cold Spring Harbor Press, New York, 1973).

    Google Scholar 

  3. Wyke, J. A., Bell, J. G., and Beamand, J. A., Cold Spring Harb. Symp. quant. Biol., 39, 897–905 (1974).

    Article  Google Scholar 

  4. Huebner, R., and Todaro, G. J., Proc. natn. Acad. Sci. U.S.A., 64, 1087–1094 (1969).

    Article  ADS  CAS  Google Scholar 

  5. Todaro, G. J., and Huebner, R. J., Proc. natn. Acad. Sci. U.S.A., 69, 1009–1015 (1972).

    Article  ADS  CAS  Google Scholar 

  6. Scolnick, E. M., Maryak, J. M., and Parks, W. P., J. Virol., 14, 1435–1444 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Lai, M. M.-C., Duesberg, P. H., Horst, J., and Vogt, P. K., Proc. natn. Acad. Sci. U.S.A., 70, 2266–2271 (1973).

    Article  ADS  CAS  Google Scholar 

  8. Stehelin, D., Guntaka, R. V., Varmus, H. E., and Bishop, J. M., J. molec. Biol., 101, 349–365 (1976).

    Article  CAS  Google Scholar 

  9. Stehelin, D., Varmus, H. E., Bishop, J. M., and Vogt, P. K., Nature, 260, 170–173 (1976).

    Article  ADS  CAS  Google Scholar 

  10. Scolnick, E. M., Goldberg, R. J., and Williams, D., J. Virol., 18, 559–566 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Frankel, A. E., and Fischinger, P. J., J. Virol. (in the press).

  12. Cohen, S., Carpenter, G., and Lembach, K. J., Adv. Metab. Disord., 8, 265–284 (1975).

    Article  CAS  Google Scholar 

  13. Cohen, S., J. biol. Chem., 237, 1555–1562 (1962).

    CAS  PubMed  Google Scholar 

  14. Cohen, S., and Taylor, J. M., in Recent Progress in Hormone Research (edit. by Creep, R. O.), 30, 533–550 (Academic, New York, 1974).

    Google Scholar 

  15. Cohen, S., and Savage, C. R., Jr, in Recent Progress in Hormone Research (edit. by Greep, R. O.), 30, 551–574 (Academic, New York, 1974).

    Google Scholar 

  16. Turkington, R. W., Expl Cell Res., 57, 69–85 (1969).

    Article  Google Scholar 

  17. Armelin, H. A., Proc. natn. Acad. Sci. U.S.A., 70, 2702–2706 (1973).

    Article  ADS  CAS  Google Scholar 

  18. Rose, S. P., Pruss, R. M., and Herschman, H. R., J. cell. Physiol., 86, 593–598 (1975).

    Article  Google Scholar 

  19. Hollenberg, M. D., and Cuatrecasas, P., Proc. natn. Acad. Sci. U.S.A., 70, 2964–2968 (1973).

    Article  ADS  CAS  Google Scholar 

  20. Westermark, B., Biochem. biophys. Res. Commun., 69, 304–310 (1976).

    Article  CAS  Google Scholar 

  21. Cohen, S., and Carpenter, G., Proc. natn. Acad. Sci. U.S.A., 72, 1317–1321 (1975).

    Article  ADS  CAS  Google Scholar 

  22. Gregory, H., Nature, 257, 325–327 (1975).

    Article  ADS  CAS  Google Scholar 

  23. Roth, J., Metabolism, 22, 1059–1073 (1973).

    Google Scholar 

  24. Carpenter, G., Lembach, K. J., Morrison, M. M., and Cohen, S., J. biol. Chem., 250 4297–4304 (1975).

    CAS  Google Scholar 

  25. Hollenberg, M. D., and Cuatrecasas, P., J. biol. Chem., 250, 3845–3853 (1975).

    CAS  PubMed  Google Scholar 

  26. Todaro, G. J., and Green, H., J. Cell Biol., 17, 299–313 (1963).

    Article  CAS  Google Scholar 

  27. Aaronson, S. A., and Todaro, G. J., Science, 162, 1024–1026 (1968).

    Article  ADS  CAS  Google Scholar 

  28. Jainchill, J. L., Aaronson, S. A., and Todaro, G. J., J. Virol., 4, 549–553 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Moloney, J. B., Symp. Zool. Soc. Lond., 17, 251–258 (1966).

    Google Scholar 

  30. Kirsten, W. H., and Mayer, L. A., J. natn. Cancer Inst., 39, 311–335 (1967).

    CAS  Google Scholar 

  31. Todaro, G. J., Green, H., and Goldberg, B. D., Proc. natn. Acad. Sci. U.S.A., 51, 66–73 (1964).

    Article  ADS  CAS  Google Scholar 

  32. Todaro, G. J., Habel, K., and Green, H., Virology, 27, 179–185 (1965).

    Article  CAS  Google Scholar 

  33. Benjamin, T. L., J. molec. Biol., 16, 359–373 (1966).

    Article  CAS  Google Scholar 

  34. Duc-Nguyen, H., Rosenblum, E. N., and Zeigel, R. F., J. Bact., 92, 1133–1140 (1966).

    Google Scholar 

  35. Henderson, I. C., Lieber, M. M., and Todaro, G. J., Virology, 60, 282–287 (1974).

    Article  CAS  Google Scholar 

  36. Todaro, G. J., Benveniste, R. E., Lieber, M. M., and Livingston, D. M., Virology, 55, 506–515 (1973).

    Article  CAS  Google Scholar 

  37. Gardner, M. B., et al., J. Am. Vet. Med. Ass., 158, 1046–1053 (1971).

    Google Scholar 

  38. Benveniste, R. E., et al., Nature, 248, 17–20 (1974).

    Article  ADS  CAS  Google Scholar 

  39. Todaro, G. J., Sherr, C. J., Benveniste, R. E., Lieber, M. M., and Melnick, J. L., Cell, 2, 55–61 (1974).

    Article  CAS  Google Scholar 

  40. Starkey, R. H., Cohen, S., and Orth, D. N., Science, 189, 800–802 (1975).

    Article  ADS  CAS  Google Scholar 

  41. De Larco, J., and Todaro, G. J., Cell, 8, 365–371 (1976).

    Article  CAS  Google Scholar 

  42. Nicolson, G. L., Lacorbiere, M., and Eckhart, W., Biochemistry, 14, 172–179 (1975).

    Article  CAS  Google Scholar 

  43. Thomopoulos, P., Roth, J., Lovelace, E., and Pastan, I., Cell, 8, 417–424 (1976).

    Article  CAS  Google Scholar 

  44. Scolnick, E. M., Howk, R. S., Anisowicz, A., Peebles, P. T., Scher, C. D., and Parks, W. P., Proc. natn. Acad. Sci. U.S.A., 72, 4650–4654 (1975).

    Article  ADS  CAS  Google Scholar 

  45. Theilen, G. H., Gould, D., Fowler, M., and Dungworth, D. L., J. natn. Cancer Inst., 47, 881–889 (1971).

    CAS  Google Scholar 

  46. Todaro, G. J., Nature new Biol., 240, 157–160 (1972).

    Article  CAS  Google Scholar 

  47. Del Villano, B. C., Nave, B., Croker, B. P., Lerner, R. A., and Dixon, F. J., J. exp. Med., 141, 172–187 (1975).

    Article  CAS  Google Scholar 

  48. Obata, Y., Ikeda, H., Stockert, E., and Boyse, E. A., J. exp. Med., 141, 188–197 (1975).

    Article  CAS  Google Scholar 

  49. Tung, J.-S., Vitetta, E. S., Fleissner, E., and Boyse, E. A., J. exp. Med., 141, 198–205 (1975).

    Article  CAS  Google Scholar 

  50. Lerner, R. A., Wilson, C. B., Del Villano, B. C., McConahey, P. J., and Dixon, F. J., J. exp. Med., 143, 151–166 (1976).

    Article  CAS  Google Scholar 

  51. Gospodarowicz, D., and Moran, J. S., J. Cell Biol., 66, 451–457 (1975).

    Article  CAS  Google Scholar 

  52. Holley, R. W., and Kiernan, J. A., Proc. natn. Acad. Sci. U.S.A., 71, 2908–2911 (1974).

    Article  ADS  CAS  Google Scholar 

  53. Levi-Montalcini, R., Meyer, H., and Hamburger, V., Cancer Res., 14, 49–57 (1954).

    CAS  PubMed  Google Scholar 

  54. Dulak, N. C., and Temin, H. M., J. cell. Physiol., 81, 153–160 (1973).

    Article  CAS  Google Scholar 

  55. Smith, G. L., and Temin, H. M., J. cell. Physiol., 84, 181–192 (1974).

    Article  CAS  Google Scholar 

  56. Rechler, M. M., Podskalny, J. M., and Nissley, S. P., Nature, 259, 134–136 (1976).

    Article  ADS  CAS  Google Scholar 

  57. O'Keefe, E., Hollenberg, M. D., and Cuatrecasas, P., Archs Biochem. Biophys., 164, 518–526 (1974).

    Article  CAS  Google Scholar 

  58. Carpenter, G., and Cohen, S., J. Cell Biol. (in the press).

Download references

Author information

Authors and Affiliations

  1. Laboratory of Viral Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    George J. Todaro & Joseph E. De Larco

  2. Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, 37203

    Stanley Cohen

Authors
  1. George J. Todaro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joseph E. De Larco
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stanley Cohen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Todaro, G., De Larco, J. & Cohen, S. Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cells. Nature 264, 26–31 (1976). https://doi.org/10.1038/264026a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/264026a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing