Abstract
TRANSLATION of parathyroid hormone (PTH) mRNA in extracts of wheat germ1 or Krebs II ascites cells2 produces a polypeptide which contains the structure of PTH but which is larger than either PTH or its biosynthetic precursor proparathyroid hormone3,4 (proPTH). In the wheat germ system, this cell-free protein, designated pre-proparathyroid hormone (pre-proPTH) contains a methiomne at the amino terminus5 derived from initiator methionyl-tRNA6. Although this observation suggests that initiation of the synthesis of pre-proPTH is normal in the cell-free system, it is possible that in vitro initiation occurs at a codon that codes for an internal methionine in vivo. Such an artefact could be due to a degraded mRNA in which the in vivo initiator codon is lost or inactivated.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kemper, B., Habener, J. F., Mulligan, R. C., Potts, J. T., Jr, and Rich, A., Proc. natn. Acad. Sci. U.S.A., 71, 3731–3735 (1974).
Habener, J. F., Kemper, B., Potts, J. T., Jr, and Rich, A., Biochem. Biophys Res. Commun., 67, 1114–1121 (1975).
Kemper, B., Habener, J. F., Potts, J. T., Jr, and Rich, A., Proc. natn. Acad. Sci. U.S.A., 69, 643–647 (1972).
Cohn, D. V., MacGregor, R. R., Chu, L. L. H., Kimmel, J. R., and Hamilton, J. W., Proc. natn. Acad. Sci. U.S.A., 69, 1521–1525 (1972).
Kemper, B., Habener, J. F., Ernst, M. D., Potts, J. T., and Rich, A., Biochemistry, 15, 15–20 (1976).
Kemper, B., Habener, J. F., Potts, J. T., Jr, and Rich, A., Biochemistry, 15, 20–25 (1976).
Both, G. W., Furuichi, Y., Muthukrishnan, S., and Shatkin, A. J., Cell, 6, 185–195 (1975).
Both, G. W., Banerjee, A. K., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 72, 1189–1193 (1975).
Muthukrishnan, S., Both, G. W., Furuichi, Y., and Shatkin, A. J., Nature, 255, 33–37 (1975).
Muthukrishnan, S., et al., J. biol. Chem., 250, 9336–9341 (1975).
Wimmer, E., Chang, A. Y., Clark, J. M., Jr, and Reichmann, M. E., J. molec. Biol., 38, 59–73 (1968).
Horst, J., Fraenkel-Conrat, H., and Mandeles, S., Biochemistry, 10, 4748–4752 (1971).
Rao, M. S., Wu, B. C., Waxman, J., and Busch, H., Biochem. biophys. Res. Commun., 66, 1186–1193 (1975).
Steinschneider, A., and Fraenkel-Conrat, H., Biochemistry, 5, 2735–2743 (1966).
Labrie, F., Nature, 221, 1217–1222 (1969).
Palmiter, R. D., J. biol. Chem., 248, 2095–2106 (1973).
Mayer, S. A., Abraham, G., Alder, R., and Banerjee, A. K., Cell, 5, 59–67 (1975).
Laemmli, U. K., Nature, 227, 680–685 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KEMPER, B. Inactivation of parathyroid hormone mRNA by treatment with periodate and aniline. Nature 262, 321–323 (1976). https://doi.org/10.1038/262321a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/262321a0
This article is cited by
-
Early origins and evolution of microRNAs and Piwi-interacting RNAs in animals
Nature (2008)
-
Efficient translation of prokaryotic mRNAs in a eukaryotic cell-free system requires addition of a cap structure
Nature (1979)
-
DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription—translation system
Nature (1977)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
