Abstract
SEVERAL workers have shown that stimulation of mouse spleen lymphocytes by E. coli lipopolysaccharide (LPS) results in extensive proliferation of B lymphocytes and the rapid maturation of immunoglobulin M (IgM)-secreting cells1–3. Not all B lymphcytes that are stimulated to DNA synthesis and division by LPS, however, develop into high rate Ig-secreting cells4,5; dividing blast cells can give rise to large numbers of non-dividing small B lymphocytes with a high density of surface Ig and no detectable intracellular Ig pool4. In these respects such secondary cells are thought to resemble memory cells. Those cells which do develop into high rate Ig-secreting cells during a few days of culture are generally restricted to secretion of IgM7,6. We report here that in improved culture conditions, and dependent on repeated cycles of mitogenic stimulation, precursors to IgG and IgA as well as IgM-secreting cells can undergo the full maturation sequence. Our results suggest that one or more phases of resting cells are intermediates in this sequence.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Greaves, M. F., and Janossy, G., Transplant. Rev., 11, 87–130 (1972).
Andersson, J., Sjöberg, O., and Möller, G., Transplant. Rev., 11, 131–177 (1972).
Melchers, F., and Andersson, J., Transplant. Rev., 14, 76–130 (1973).
Askonas, B. A., and Roelants, G. E., Eur. J. Immun. (in the press).
Janossy, G., and Greaves, M. F., Transplant. Rev., 24, 177–236 (1975).
Parkhouse, R. M. E., Janossy, G., and Greaves, M. F., Nature new Biol., 235, 21–23 (1972).
Andersson, J., and Melchers, F., Proc. natn. Acad. Sci. U.S.A., 70, 416–420 (1973).
Askonas, B. A., Schimpl, A., and Wecker, E., Eur. J. Immun., 4, 164–169 (1974).
Parkhouse, R. M. E., and Askonas, B. A., Biochem. J., 115, 163–169 (1969).
Maizel, J. V., in Methods in Virology, 5 (edit. by Maramorosch, K., and Koprowski, H.), 180–247 (Academic, New York, 1971).
Raff, M. C., Nature, 226, 1257–1258 (1970).
North, J. R., and Askonas, B. A., Eur. J. Immun., 6, 8–15 (1976).
Dresser, D. W., and Greaves, M. F., in Handbook of Experimental Immunology (edit. by Weir, D. M.), Ch. 27, 1–22 (Blackwell, Oxford, 1973).
Kreisler, J. M., and Möller, G., J. Immun., 112, 151–161 (1974).
Amerding, D., and Katz, D. H., J. exp. Med., 139, 24–43 (1974).
Holtzer, H., Weintraub, H., Mayne, R., and Mochan, B., in Curr. Topics devl Biol., 7, 229–256 (1973).
Levitt, D., and Dorfman, A., in Curr. Topics devl Biol., 8, 103–149 (1974).
Kearney, J. F., and Lawton, A. R., J. Immun., 115, 671–676 (1975).
Melchers, F., Continho, A., Heinrich, G., and Andersson, J., Scand. J. Immun., 4, 853–858 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ZAUDERER, M., ASKONAS, B. Several proliferate phases precede maturation of IgG-secreting cells in mitogen-stimulated cultures. Nature 260, 611–613 (1976). https://doi.org/10.1038/260611a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/260611a0
This article is cited by
-
Optimal strategies in immunology III. The IgM-IgG switch
Journal of Mathematical Biology (1980)
-
IgM-IgG switch-over among antibody-forming cells in the mouse
Nature (1977)
-
Immunodepression, high IgM levels and evasion of the immune response in murine trypanosomiasis
Nature (1976)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
