Abstract
DENERVATION of skeletal muscles causes a spread of acetyl-choline (ACh) sensitivity along muscle fibres, but it is not yet clear whether this results from muscle inactivity or from a loss of trophic influence of the nerve on the muscle1. It has been suggested that inactivity is responsible for the phenomenon because the application of a cuff containing a local anaesthetic to a nerve blocked the conduction of the nerve impulses and caused the development of widespread sensitivity to ACh (ref. 2, but see ref. 3). The local anaesthetics used in that study, however, have since been shown to block axoplasmic transport in vitro4,5 and in vivo6, as well as to induce neuronal degeneration7. Axoplasmic transport seems to be involved in the trophic influence of the nerve on muscle because blocking this transport induces spreading of ACh sensitivity in muscle fibres8,9. We have used tetrodotoxin (TTX), incorporated into silicone cuffs placed around motor nerves, to test whether the resultant inactivity induced a spread of ACh sensitivity in skeletal muscle, as inferred from the binding of α-bungarotoxin (α-BuTX). TTX was chosen because it does not block axoplasmic transport in vitro5.
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LAVOIE, PA., COLLIER, B. & TENENHOUSE, A. Comparison of α-bungarotoxin binding to skeletal muscles after inactivity or denervation. Nature 260, 349–350 (1976). https://doi.org/10.1038/260349a0
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DOI: https://doi.org/10.1038/260349a0
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