Abstract
RECENT structural studies1,2 indicate that the DNA of filamentous bacteriophages is enclosed in a sheath of α-helical coat protein. In the course of phage biosynthesis this protein is tightly bound to the membrane of the host (Escherichia coli)3,4. We present here the results of circular dichroism (CD) studies strongly suggesting that the protein has a quite different conformation in its membrane-bound state, so that a major conformational change must accompany incorporation into the virus.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Marvin, D. A., Pigram, W. J., Wiseman, R. L., Wachtel, E. J., and Marvin, F. J., J. molec. Biol., 88, 581–598 (1974).
Marvin, D. A., and Wachtel, E. J., Nature, 253, 19–23 (1975).
Smilowitz, H., Carson, J., and Robbins, P. W., J. supramolec. Struct., 1, 8–18 (1972).
Webster, R. E., and Cashman, J. S., Virology, 55, 20–38 (1973).
Makino, S., Woolford, J. L., Jr, Tanford, C., and Webster, R. E., J. biol. Chem., 250, 4327–4332 (1975).
Bush, C. A., in Basic Principles in Nucleic Acid Chemistry, II, (edit. by Ts'o, P. O. P.), 145–151 (Academic, New York, 1974).
Wells, B. D., and Yang, J. T., Biochemistry, 13, 1317–1321 (1974).
Hanlon, S., Brudno, S., Wu, T. T., and Wolf, B., Biochemistry, 14, 1648–1660 (1975).
Marvin, D. A., and Hohn, B., Bact. Rev., 33, 172–209 (1969).
Huang, C., Biochemistry, 8, 344–352 (1969).
Greenfield, N., and Fasman, G. D., Biochemistry, 8, 4108–4116 (1969).
Chen, Y. H., Yang, J. T., and Martinez, H. M., Biochemistry, 11, 4120–4131 (1972).
Holzwarth, G., in Membrane Molecular Biology (edit. by Fox, C. F., and Keith, A. D.), 228–286 (Sinauer Associates, Stamford, Connecticut, 1974).
Litman, B. J., Biochemistry, 11, 3243 (1972).
Day, L. A., J. molec. Biol., 15, 395–398 (1966); 39, 265–277 (1969).
Asbeck, F., Bayereuther, K., Köhler, H., von Wettstein, G., and Braunitzer, G., Hoppe-Seyler's Z. Physiol. Chem., 350, 1047–1066 (1969).
Nakashima, Y., and Konigsberg, W., J. molec. Biol., 88, 598–600 (1974).
Chou, P. Y., and Fasman, G. D., Biochemistry, 13, 211–222, 222–245 (1974).
Krigbaum, W. R., and Knutton, S. P., Proc. natn. Acad. Sci. U.S.A., 70, 2809–2813 (1973).
Woolford, J. L., Jr, and Webster, R. E., J. biol. Chem., 250, 4333–4339 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
NOZAKI, Y., CHAMBERLAIN, B., WEBSTER, R. et al. Evidence for a major conformational change of coat protein in assembly of fl bacteriophage. Nature 259, 335–337 (1976). https://doi.org/10.1038/259335a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/259335a0
This article is cited by
-
Analysis of time-resolved fluorescence anisotropy in lipid-protein systems
European Biophysics Journal (1990)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.