Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Interaction of multiplication-stimulating activity with chick embryo fibroblasts demonstrates a growth receptor

Nature volume 259, pages 134–136 (1976)Cite this article

Abstract

MULTIPLICATION-STIMULATING activity (MSA), which is the name given to a family of polypeptides isolated from calf serum1 and from medium conditioned by certain rat liver cell cultures2, stimulates cellular DNA synthesis and growth, and has insulin-like activity. MSA is acid soluble, heat stable and has a molecular weight of approximately 10,0001,2. It shares these physical and biological properties with somatomedin3 and acid–ethanol soluble non-suppressible insulin-like activity (NSILA-s)4—polypeptides in human serum which may be important in human growth. We have now studied the interaction of MSA and insulin with chick embryo fibroblasts (CEF). We describe here the binding of labelled MSA to CEF, and correlate this binding with the stimulation of incorporation of 3H-thymidine into DNA. We propose that MSA and insulin stimulate DNA synthesis in CEF by interacting with a common growth receptor.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Pierson, R. W., Jr, and Temin, H. M., J. cell. Physiol., 79, 319–330 (1972).

    Article  CAS  Google Scholar 

  2. Dulak, N. C., and Temin, H. M., J. cell. Physiol., 81, 153–160 (1973); ibid., 81, 161–170 (1973).

    Article  CAS  Google Scholar 

  3. Van Wyk, J. J., et al., Rec. Prog. Horm. Res., 30, 259–295 (1974).

    CAS  PubMed  Google Scholar 

  4. Oelz, O., Froesch, E. R., Bünzli, H. F., Humbel, R. E., and Ritschard, W. J., in Handbook of Physiology (edit. by Steiner, D. F. and Freinkel, N.). 1, 685–702 (American Physiological Society, Washington DC, 1972).

    Google Scholar 

  5. Temin, H. M., in Growth Regulating Substances for Animal Cells in Culture, (edit. by Defendi, V. and Stoker, M.), Wistar Inst. Symp. Monog. No. 7, 103–114 (Wistar Institute Press, Philadelphia, 1967).

    Google Scholar 

  6. Cohen, K. L., Short, P. A., and Nissley, S. P., Endocrinology, 96, 193–198 (1975).

    Article  CAS  Google Scholar 

  7. Smith, G. L., and Temin, H. M., J. cell. Physiol., 84, 181–192 (1974).

    Article  CAS  Google Scholar 

  8. Rechler, M. M., Podskalny, J. M., Goldfine, I. D., and Wells, C. A., J. clin. Endocr. Metab., 39, 512–521 (1974).

    Article  CAS  Google Scholar 

  9. Holley, R. W., and Kiernan, J. A., Proc. natn. Acad. Sci. U.S.A., 71, 2908–2911 (1974).

    Article  ADS  CAS  Google Scholar 

  10. Roth, J., Metabolism, 22, 1059–1073 (1973).

    Article  CAS  Google Scholar 

  11. Megyesi, K., et al., Biochem. biophys. Res. Commun., 57, 307–315 (1974).

    Article  CAS  Google Scholar 

  12. Podskalny, J. M., Chou, J. Y., and Rechler, M. M., Archs Biochem. Biophys., 170, 504–513 (1975).

    Article  CAS  Google Scholar 

  13. Freychet, P., Roth, J., and Neville, D. M., Jr, Proc. natn. Acad. Sci. U.S.A., 68, 1833–1837 (1971).

    Article  ADS  CAS  Google Scholar 

  14. Gavin, J. R., III, Gorden, P., Roth, J., Archer, J. A., and Buell, D. N., J. biol. Chem., 248, 2202–2207 (1973).

    CAS  Google Scholar 

  15. De Meyts, P., Roth, J., Neville, D. M., Jr, Gavin, J. R., III, and Lesniak, M. A., Biochem. biophys. Res. Commun., 55, 154–161 (1973).

    Article  CAS  Google Scholar 

  16. Zaph, J., Mäder, M., Waldvogel, M., Schalch, D. S., and Froesch, E. R., Archs Biochem. Biophys., 168, 630–637 (1975).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Diabetes Branch, National Institute of Arthritis, Metabolism and Digestive Diseases,

    MATTHEW M. RECHLER & JUDY M. PODSKALNY

  2. Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    S. PETER NISSLEY

Authors
  1. MATTHEW M. RECHLER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. JUDY M. PODSKALNY
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. PETER NISSLEY
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

RECHLER, M., PODSKALNY, J. & NISSLEY, S. Interaction of multiplication-stimulating activity with chick embryo fibroblasts demonstrates a growth receptor. Nature 259, 134–136 (1976). https://doi.org/10.1038/259134a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/259134a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing