Abstract
THE yield of radiation-induced strand breaks in DNA is in normal conditions, larger in oxygenated cells than in anoxic cells. This may reflect either radiochemical reactions or preferential repair of breaks formed in anoxia. It is possible to distinguish between these possibilities by experiments in which irradiation and cell lysis are completed within a time period sufficiently short to minimise repair. We have measured in oxic and anoxic cells the yield of strand breakage on bacteriophage λ DNA superinfecting lysogenic bacteria1 and now on chromosomal DNA in experiments where irradiation and lysis are carried out within a fraction of a second.
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JOHANSEN, I., BOYE, E. Radiation-induced DNA strand breaks in E. coli measured within a fraction of a second. Nature 255, 740–742 (1975). https://doi.org/10.1038/255740a0
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DOI: https://doi.org/10.1038/255740a0
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