Abstract
A STEPWISE accumulation of individual enzyme activities is a common feature of the eukaryotic cell cycle1. Two major hypotheses consider that the temporal restriction is produced by oscillatory repression or by linear reading of genes along the chromosome2,3. Both hypotheses, in their present form, assume that increase in enzyme activity is concurrent with transcription and translation. We have made use of the high degree of synchrony obtainable with Chlorella fusca var. vacuolata 211-8p, grown under intermittent illumination4,5, to study the duration of transcription and translation for succinate dehydrogenase by applying inhibitors during the period of its stepwise increase in activity. We report that the periods of transcription, of translation and of increase in activity are only partially concurrent.
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FORDE, B., JOHN, P. Transcription, translation and maturation of succinate dehydrogenase during cell cycle. Nature 252, 410–412 (1974). https://doi.org/10.1038/252410a0
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DOI: https://doi.org/10.1038/252410a0
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