Abstract
IN eukaryotic cells, the DNA-dependent RNA polymerase is known to be bound to chromatin1. As it is known that chromatin is a restricted template2,3, the question is whether all or only a fraction of the RNA polymerases bound to the chromatin is functionally active. The experiments reported here provide evidence that only a fraction of the RNA polymerases present in both nuclei and the nucleolar fraction is functionally active on chromatin.
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References
Weiss, S. B., Proc. natn. Acad. Sci. U.S.A., 46, 1020–1030 (1960).
Bonner, J., Dahmus, M. E., Fambrough, D., Huang, R. C. C., Marushige, K., and Tuan, D. Y. H., Science, 159, 47–56 (1968).
Paul, J., and Gilmour, R. S., J. molec. Biol., 34, 305–316 (1968).
Yu, F. L., and Feigelson, P., Proc. natn. Acad. Sci. U.S.A., 68, 2177–2180 (1971); 69 2833–2837 (1972).
Reich, E., and Goldberg, I. H., Prog. Nucleic Acid Res. molec. Biol., 3, 183–234 (1964).
Roeder, R. G., and Rutter, W. J., Nature, 224, 234–237 (1969).
Jacob, S. T., Sajdel, E. M., Muecke, W., and Munro, H. N., Cold Spring Harbor Symp. quant. Biol., 35, 681–691 (1970).
Lindell, T. J., Weinberg, F., Morris, P. W., Roeder, R. G., and Rutter, W. J., Science, 170, 447–449 (1970).
Kedinger, C., Gnaizdowski, M., Mandel, J. L., jun., Gissinger, F., and Chambon, P., Biochem. biophys. Res Commun., 38, 165–171 (1970).
Bray, G. A., Analyt. Biochem., 1, 279–285 (1960).
Burton, K., Meth. Enzymol., 12(B), 163–166 (1968).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, J. R., J. biol. Chem., 193, 265–275 (1951).
Roeder, R. G., and Rutter, W. J., Proc. natn. Acad. Sci. U.S.A., 65, 675–682 (1970).
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YU, FL. Two functional states of the RNA polymerases in the rat hepatic nuclear and nucleolar fractions. Nature 251, 344–346 (1974). https://doi.org/10.1038/251344a0
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DOI: https://doi.org/10.1038/251344a0
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