Abstract
MANY assays for alloantibody depend on functional activities of the Fc portion of the immunoglobulin molecule. These functional activities are acquired when the antibody binds to its target cell. Complement binding, as demonstrated by lysis or complement fixation, has been the most widely used of these activities. In man, alloantibody can also be detected by its ability to mediate cell-dependent lysis of target cells1–3. In this system, effector cells (human peripheral blood leukocytes) lyse allogeneic chromium 51Cr-labelled target cells (lymphocytes) in the presence of the appropriate alloantibody, providing a sensitive, complement-independent assay for alloantibody. In contrast, we have found that mouse alloantisera do not usually mediate lysis of mouse target cells (lymph node lymphocytes, phytohaemagglutinin (PHA) blast cells, and tumour cells) by mouse effector cells (spleen cells). Data supporting this conclusion will be presented elsewhere.
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HALLORAN, P., FESTENSTEIN, H. Inhibition of cell-dependent cytotoxicity as an assay for mouse alloantibody. Nature 250, 52–54 (1974). https://doi.org/10.1038/250052a0
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DOI: https://doi.org/10.1038/250052a0
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