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Photophosphorylation of an ADP analogue by spinach chloroplasts

Abstract

THE mechanism of ATP synthesis during photosynthetic phosphorylation is not yet known. It has been shown, however, that a coupling factor protein is necessary for coupling ATP synthesis to electron transport1. An interesting mechanism for photophosphorylation has been proposed2,3 whereby photosynthetic formation of ATP is brought about by transphosphorylation of two coupling factor-bound ADP molecules to form AMP and ATP. The AMP would then be rephosphorylated in a step dependent on electron transport to form coupling factor-bound ADP which would act as the high energy intermediate (which has been thought to exist) to phosphorylate another ADP molecule to ATP.

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HORAK, A., ZALIK, S. Photophosphorylation of an ADP analogue by spinach chloroplasts. Nature 249, 858–860 (1974). https://doi.org/10.1038/249858a0

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