Abstract
GENETIC analysis of i gene mutants which had lost the capacity to bind operator but not inducer suggested that lac repressor binds with its amino terminus to lac operator1. Here we will show that the carboxy terminus of lac repressor is dispensable: We found fusions between the i (lac repressor) and z (β-galactosidase) genes (Fig. 1) which produce fully active lac repressor covalently linked to active β-galactosidase.
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MüLLER-HILL, B., KANIA, J. Lac repressor can be fused to β-galactosidase. Nature 249, 561–563 (1974). https://doi.org/10.1038/249561a0
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DOI: https://doi.org/10.1038/249561a0
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