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Relationship between tritiated poly-L-lysine binding and template activity of human chromosomes

Nature volume 249, pages 559–561 (1974)Cite this article

Abstract

IT has been reported that cytological preparations of chromosomes can serve as templates in RNA polymerase reactions1–2. The newly synthesised RNA on chromosomes was readily digested with RNase in contrast to RNA which was synthesised in a similar manner but using as a template denatured DNA immobilised on nitrocellulose filters1. When cytological preparations of chromosomes were pretreated with poly-L-lysine (PL), no synthesis of RNA could be demonstrated2. The implication was that bound PL blocked those sites on DNA template which were responsible for transcription.

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Authors and Affiliations

  1. Department of Biological Sciences, State University of New York at Albany, Albany, New York, 12222

    S. P. MARFEY & M. G. LI

Authors
  1. S. P. MARFEY
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  2. M. G. LI
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MARFEY, S., LI, M. Relationship between tritiated poly-L-lysine binding and template activity of human chromosomes. Nature 249, 559–561 (1974). https://doi.org/10.1038/249559a0

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