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Infrared Zimm plots for macromolecular characterisation

Abstract

THE light scattering method is an accepted procedure for the measurement of the molecular weight (M) and the size of macromolecules in solution. This information is most readily available for particles the major dimension (ζ) of which is of the order of the wavelength (λ) of the light incident on the system. Provided that they are not too optically dense, such particles satisfy the conditions and are described in terms of the Rayleigh–Gans–Debye1,2 (RGD) scattering theory. In this equation, m is the ratio of the refractive index (n) of the solute to that of the solvent (n0).

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MORRIS, V., COLES, H. & JENNINGS, B. Infrared Zimm plots for macromolecular characterisation. Nature 249, 240–241 (1974). https://doi.org/10.1038/249240a0

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