Abstract
THE use of cell hybridisation for complementation tests is hampered because the establishment of a hybrid cell line may involve rearrangement of both parental cells' genetic material, and often loss of chromosomes1–3. Since it is difficult to recognise single chromosomes, the genotype of the hybrid cannot be determined easily. Furthermore, strong selective pressures are often applied to isolate hybrid cells. Thus, the appearance of the wild type phenotype in a hybrid between a mutant and a wild type cell does not always imply that the mutation is recessive and can be complemented by the wild type allele: alternative hypotheses must be considered.
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TONIOLO, D., BASILICO, C. Complementation of a defect in the production of ribosomal RNA in somatic cell hybrids. Nature 248, 411–413 (1974). https://doi.org/10.1038/248411a0
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DOI: https://doi.org/10.1038/248411a0
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