Abstract
SPERMATOZOA mature while passing through the epididymis. In rabbit, fertilizing ability is acquired when spermatozoa pass through the corpus epididymidis1,2. Spermatozoa retained by ligatures in the proximal corpus develop a normal fertilizing ability in 24 h in intact males3,4, but not in castrated males5 or when the proximal corpus is maintained for 24 h in vitro in organ culture6. Spermatozoa from a ligated proximal corpus of a castrated male become fertile only when exogenous testosterone is given5. This indicates that rabbit sperm maturation is testosterone dependent. A technique for maintaining viable spermatozoa in epididymal segments in organ culture in vitro has been described6. Here we report the effect on the fertilizing ability of spermatozoa from the rabbit proximal corpus of adding testosterone to the culture medium.
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ORGEBIN-CRIST, M., TICHENOR, P. Effect of Testosterone on Sperm Maturation in vitro. Nature 245, 328–329 (1973). https://doi.org/10.1038/245328a0
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DOI: https://doi.org/10.1038/245328a0
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