Abstract
IT has been reported that cytochalasin B can prevent cytokinesis without influencing nuclear division1. Cells undergoing mitosis in the presence of cytochalasin B become binucleate and occasionally, with prolonged incubation, multinucleate1–3. Treated cells generally resume cleavage upon removal from cytochalasin and reculturing. It has been shown that many of the binucleate cells produced in a BHK21 cell line will develop as tetraploids upon reculturing in fresh medium4,5. Here I report the treatment of cleaving mouse eggs with cytochalasin and the establishment of tetraploidy in mouse embryos.
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SNOW, M. Tetraploid Mouse Embryos produced by Cytochalasin B during Cleavage. Nature 244, 513–515 (1973). https://doi.org/10.1038/244513a0
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DOI: https://doi.org/10.1038/244513a0
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