Abstract
DURING protein synthesis in bacteria, aminoacyl tRNA is transported to the ribosome complexed with a protein elongation factor Tu (EF-TU) and GTP1–3. In the ribosome the aminoacyl tRNA is positioned onto its appropriate codon, GTP is hydrolysed, and EF-Tu is released as a stable complex with GDP. The EF-Tu·GDP complex is then reconverted to EF-Tu·GTP by means of another soluble protein, EF-Ts. Transfer factors analogous to EF-Tu but with different physical properties have also been isolated from eucaryotic systems. The EF-Tu·GDP complex has been purified to homogeneity from Escherichia coli4,5. The protein factor has a molecular weight around 40,000 and is composed of a single polypeptide chain. It forms a stable 1 : 1 complex with GDP which has a dissociation constant of 3 × 10−9 M4. In the purification of EF-Tu from E. coli, needle shaped microcrystals have been reported4,5 on addition of ammonium sulphate; these crystals were too small for X-ray diffraction analysis, however. Here we report the preparation of large crystals of an EF-Tu·GDP complex and describe their preliminary analysis by X-ray diffraction.
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References
Lucas-Lenard, J., and Lipmann, F., Ann. Rev. Biochem., 40, 490 (1971).
Gordon, J., Proc. US Nat. Acad. Sci., 58, 1574 (1967).
Shorey, R. L., Ravel, J. M., Garner, C. W., and Shive, W., J. Biol. Chem., 244, 4555 (1969).
Miller, D. L., and Weissbach, H., Arch. Biochem. Biophys., 141, 26 (1970).
Parmeggiani, A., and Goltschalk, E. M., Cold Spring Harbor Symp Quant. Biol., 34, 377 (1969).
Kim, S. H., Quigley, G., Suddath, F. L., and Rich, A., Proc. US Nat. Acad. Sci., 68, 841 (1971).
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SNEDEN, D., MILLER, D., KIM, S. et al. Preliminary X-ray Analysis of the Crystalline Complex between Poypeptide Chain Elongation Factor, Tu, and GDP. Nature 241, 530–531 (1973). https://doi.org/10.1038/241530a0
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DOI: https://doi.org/10.1038/241530a0
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