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Acute Non Lymphocytic Leukemia

Cell line OCI/AML3 bears exon-12 NPM gene mutation-A and cytoplasmic expression of nucleophosmin

Leukemia volume 19, pages 1760–1767 (2005)Cite this article

Abstract

We recently identified a new acute myeloid leukemia (AML) subtype characterized by mutations at exon-12 of the nucleophosmin (NPM) gene and aberrant cytoplasmic expression of NPM protein (NPMc+). NPMc+ AML accounts for about 35% of adult AML and it is associated with normal karyotype, wide morphological spectrum, CD34-negativity, high frequency of FLT3-ITD mutations and good response to induction therapy. In an attempt to identify a human cell line to serve as a model for the in vitro study of NPMc+ AML, we screened 79 myeloid cell lines for mutations at exon-12 of NPM. One of these cell lines, OCI/AML3, showed a TCTG duplication at exon-12 of NPM. This mutation corresponds to the type A, the NPM mutation most frequently observed in primary NPMc+ AML. OCI/AML3 cells also displayed typical phenotypic features of NPMc+ AML, that is, expression of macrophage markers and lack of CD34, and the immunocytochemical hallmark of this leukemia subtype, that is, the aberrant cytoplasmic expression of NPM. The OCI/AML3 cell line easily engrafts in NOD/SCID mice and maintains in the animals the typical features of NPMc+ AML, such as the NPM cytoplasmic expression. For all these reasons, the OCI/AML3 cell line represents a remarkable tool for biomolecular studies of NPMc+ AML.

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Acknowledgements

This work was supported by the Associazione Italiana per la Ricerca sul Cancro (AIRC). We thank Claudia Tibidò for the assistance in the preparation of the manuscript. We are indebted to Roberta Pacini and Alessia Tabarrini for performing the immunohistochemical studies.

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Authors and Affiliations

  1. DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

    H Quentmeier, W G Dirks, R A F MacLeod & H G Drexler

  2. Institute of Hematology, University of Bari, Policlinico, Bari, Italy

    M P Martelli

  3. Institute of Hematology, Policlinico, Monteluce, Perugia, Italy

    N Bolli, A Pucciarini, B Bigerna, M F Martelli, C Mecucci & B Falini

  4. Institute of Hematology, University of Foggia, Foggia, Italy

    A Liso

  5. Institute of Internal Medicine, Perugia, Policlinico, Monteluce, Italy

    I Nicoletti & R Mannucci

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  1. H Quentmeier
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  2. M P Martelli
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  3. W G Dirks
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  4. N Bolli
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  5. A Liso
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  6. R A F MacLeod
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  7. I Nicoletti
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  8. R Mannucci
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  9. A Pucciarini
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  10. B Bigerna
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  11. M F Martelli
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  12. C Mecucci
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  13. H G Drexler
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  14. B Falini
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Correspondence to B Falini.

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Quentmeier, H., Martelli, M., Dirks, W. et al. Cell line OCI/AML3 bears exon-12 NPM gene mutation-A and cytoplasmic expression of nucleophosmin. Leukemia 19, 1760–1767 (2005). https://doi.org/10.1038/sj.leu.2403899

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  • DOI: https://doi.org/10.1038/sj.leu.2403899

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